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三色堇质体小球的组成和分子结构。

Composition and molecular structure of chromoplast globules of Viola tricolor.

机构信息

Institut für Biologie II, Zellbiologie, Universität Freiburg, Schänzlestraße 1, D-7800, Freiburg i. Br., Federal Republic of Germany.

出版信息

Plant Cell Rep. 1982 Apr;1(3):111-4. doi: 10.1007/BF00272366.

DOI:10.1007/BF00272366
PMID:24259022
Abstract

Plastoglobules have been isolated in pure form from petals of the pansy, Viola tricolor L. Their chemical composition has been determined up to a recovery of 96% dry weight. Triacyl glycerols (57%) as well as carotenoids and their esters (23%) are the main constituents. Polar lipids, proteins, alkanes, phytyl esters, plastid quinones, and steryl esters have been detected in smaller amounts (cf. Table 1). The mean diameter of chromoplast globules is 280±70 nm (corresponding to a volume of 11.7×10(6) nm(3)), their buoyant density 0.93 g cm(-3). The plastoglobules are devoid of a surrounding unit membrane. However, electron microscopical evidence and analytical data are consistent with a structural model envisaging the globules to consist mainly of an apolar core, covered by a 'half unit membrane' of polar constituents.

摘要

已从三色堇花瓣中纯态分离出质体小球体。已确定其化学组成,回收率达干重的 96%。三酰基甘油(57%)以及类胡萝卜素及其酯(23%)是主要成分。较小含量下已检测到极性脂类、蛋白质、链烷烃、植基酯、质体醌和甾醇酯(参见表 1)。有色体小球体的平均直径为 280±70nm(相当于 11.7×10(6)nm(3)的体积),其浮力密度为 0.93gcm(-3)。质体小球体没有周围的单位膜。然而,电子显微镜证据和分析数据与一个结构模型一致,该模型设想小球体主要由非极性核心组成,由极性成分的“半单位膜”覆盖。

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2
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本文引用的文献

1
[The localization of plastidquinones and carotenoids in the chromoplasts of petals from sarothamnus scoparius (L.) wimm ex koch].[沙棘(Sarothamnus scoparius (L.) Wimm ex Koch)花瓣有色体中质体醌和类胡萝卜素的定位]
Planta. 1970 Jun;90(2):142-52. doi: 10.1007/BF00388042.
2
[Light dependence of phytol accumulation. A contribution to the question of chlorophyll biosynthesis].[叶绿醇积累的光依赖性。对叶绿素生物合成问题的一项贡献]
Planta. 1976 Jan;130(2):151-8. doi: 10.1007/BF00384413.
3
The colorimetric micro-determination of long-chain fatty acids.
油棕(Bactris gasipaes Kunth)果实球状有色体中脂溶性的γ-胡萝卜素、β-胡萝卜素和番茄红素。
Planta. 2014 Nov;240(5):1037-50. doi: 10.1007/s00425-014-2121-3. Epub 2014 Jul 15.
4
Composition and function of plastoglobuli : I. Isolation and purification from chloroplasts and chromoplasts.质体小球的组成和功能:I. 从叶绿体和有色体中分离和纯化。
Planta. 1985 Feb;163(2):201-7. doi: 10.1007/BF00393507.
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Composition and function of plastoglobuli : II. Lipid composition of leaves and plastoglobuli during beech leaf senescence.质体小球的组成和功能:II. 山毛榉叶片和质体小球在叶片衰老过程中的脂质组成。
Planta. 1985 Jan;163(1):91-6. doi: 10.1007/BF00395902.
6
Chromoplasts of Palisota barteri, and the molecular structure of chromoplast tubules.巴氏变色体和变色体小管的分子结构。
Planta. 1986 Jun;168(2):167-74. doi: 10.1007/BF00402960.
7
Carotenoid isomerase is key determinant of petal color of Calendula officinalis.类胡萝卜素异构酶是金盏花花瓣颜色的关键决定因素。
J Biol Chem. 2012 Jan 2;287(1):276-285. doi: 10.1074/jbc.M111.300301. Epub 2011 Nov 8.
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Carotenoid composition and carotenogenic gene expression during Ipomoea petal development.在番薯花瓣发育过程中类胡萝卜素组成和类胡萝卜素生物合成基因表达。
J Exp Bot. 2010 Mar;61(3):709-19. doi: 10.1093/jxb/erp335. Epub 2009 Nov 20.
9
Plastoglobules are lipoprotein subcompartments of the chloroplast that are permanently coupled to thylakoid membranes and contain biosynthetic enzymes.质体小球是叶绿体的脂蛋白亚区室,与类囊体膜永久相连并含有生物合成酶。
Plant Cell. 2006 Jul;18(7):1693-703. doi: 10.1105/tpc.105.039859. Epub 2006 May 26.
10
Neutral lipid bodies in prokaryotes: recent insights into structure, formation, and relationship to eukaryotic lipid depots.原核生物中的中性脂质体:关于结构、形成以及与真核生物脂质库关系的最新见解
J Bacteriol. 2005 Jun;187(11):3607-19. doi: 10.1128/JB.187.11.3607-3619.2005.
长链脂肪酸的比色微量测定法。
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4
Determination of glycerol in phosphatides.磷脂中甘油的测定
Biochim Biophys Acta. 1962 Jan 29;56:367-9. doi: 10.1016/0006-3002(62)90580-2.
5
[Chlorophyll lethals & submicroscopic morphological changes in plastids].[叶绿素致死因素与质体的亚显微形态变化]
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J Biol Chem. 1957 May;226(1):497-509.
7
A new micro method for determination of cholesterol in serum.一种测定血清中胆固醇的新微量方法。
Anal Biochem. 1968 Jul;24(1):27-33. doi: 10.1016/0003-2697(68)90056-0.
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Phospholipid analysis on a micro scale.微量磷脂分析
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[Phytol in yellowed leaves].[黄叶中的叶绿醇]
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