Department of Botany, North Carolina State University, 27695, Raleigh, North Carolina.
J Chem Ecol. 1991 Jun;17(6):1045-68. doi: 10.1007/BF01402933.
Soil core (0-2.5 and/or 0-10 cm) samples were taken from wheat no till, wheat-conventional till, and fallow-conventional till soybean cropping systems from July to October of 1989 and extracted with water in an autoclave. The soil extracts were analyzed for seven common phenolic acids (p-coumaric, vanillic,p-hydroxybenzoic, syringic, caffeic, ferulic, and sinapic; in order of importance) by high-performance liquid chromatography. The highest concentration observed was 4 μg/g soil forp-coumaric acid. Folin & Ciocalteu's phenol reagent was used to determine total phenolic acid content. Total phenolic acid content of 0- to 2.5-cm core samples was approximately 34% higher than that of the 0- to 10-cm core samples. Phenolic acid content of 0- to 2.5-cm core samples from wheat-no till systems was significantly higher than those from all other cropping systems. Individual phenolic acids and total phenolic acid content of soils were highly correlated. The last two observations were confirmed by principal component analysis. The concentrations were confirmed by principal component analysis, tions of individual phenolic acids extracted from soil samples were related to soil pH, water content of soil samples, total soil carbon, and total soil nitrogen. Indirect evidence suggested that phenolic acids recovered by the water-autoclave procedure used came primarily from bound forms in the soil samples.
1989 年 7 月至 10 月期间，从免耕小麦、常规耕作小麦和常规耕作大豆休闲轮作系统中采集了 0-2.5 和/或 0-10 厘米的土壤芯（柱）样本，并在高压釜中用水提取。用高效液相色谱法对土壤提取物中的七种常见酚酸（对香豆酸、香草酸、对羟基苯甲酸、丁香酸、咖啡酸、阿魏酸和芥子酸；按重要性顺序）进行了分析。观察到的最高浓度为 4μg/g 土壤的对香豆酸。福林-西卡尔特酚试剂用于测定总酚酸含量。0-2.5 厘米芯样品的总酚酸含量比 0-10 厘米芯样品高约 34%。免耕小麦系统 0-2.5 厘米芯样品中的酚酸含量明显高于其他所有耕作系统。土壤中酚酸的含量与总酚酸含量高度相关。最后两个观察结果通过主成分分析得到了证实。通过主成分分析证实了各酚酸的浓度，从土壤样品中提取的酚酸的浓度与土壤 pH 值、土壤样品的含水量、土壤总碳和土壤总氮有关。间接证据表明，水-高压釜法提取的酚酸主要来自土壤样品中的结合态。
