Seção de Fisiologia e Bioquimica de Plantas, Instituto de Botánica de São Paulo, C.P. 4005, SP 01051, São Paulo, Brazil.
J Chem Ecol. 1991 Jun;17(6):1079-90. doi: 10.1007/BF01402935.
Phytoalexin responses were measured by modified drop-diffusate and facilitated diffusion techniques after fungal inoculation of leaves of 32 Rubiaceae species from Brazilian forest and savanna. Such responses presented a trend similar to that previously observed for a broad sample of dicotyledonous plants and are more frequently positive for the more primitive (or slower growing) trees than for the advanced (or faster growing) herbs. Fifteen of these species analyzed during a one-year period showed that positive phytoalexin responses are stronger for the rainy (and hotter) than for the dry (and cooler) season. Species that contain relatively large quantities of phenolics gave invariably negative responses. Positive responses are not necessarily associated with the appearance of new substances within leaf tissue and are thus caused by inhibitins rather than by phytoalexins. These results are discussed recognizing that the tested plants are subject to the multifarious influences of their natural environment and of a possible conjugate-caused compartmentation of plant metabolites.
在巴西森林和草原的 32 种茜草科植物叶片接种真菌后，采用改良的点滴扩散和促进扩散技术来测量植物抗毒素反应。这些反应呈现出与先前对广泛的双子叶植物样本观察到的趋势相似，并且对于较原始（或生长较慢）的树木比先进（或生长较快）的草本植物更常呈阳性。在一年的时间里对其中的 15 种进行分析表明，阳性植物抗毒素反应在雨季（较热）比在旱季（较冷）更强。含有相对大量酚类物质的物种总是给出阴性反应。阳性反应不一定与叶组织内新物质的出现有关，因此是由抑制剂引起的，而不是由植物抗毒素引起的。在认识到所测试的植物受到其自然环境的多种影响以及植物代谢物可能共轭引起的区室化的情况下，讨论了这些结果。
