College of Pharmaceutical Sciences, Soochow University, Suzhou, China.
J Sep Sci. 2014 Feb;37(3):314-22. doi: 10.1002/jssc.201300601. Epub 2013 Dec 17.
Gardenia fruits contain valuable natural food colorants including crocins (gardenia yellow) and geniposide. In this study, a process for the enrichment of crocins and geniposide simultaneously from gardenia fruits was developed using macroporous resin and RP chromatography. The performance of eight different types of macroporous resins was evaluated. Static absorption/desorption experiments revealed that LX60 possessed optimal separating capacity. Further dynamic absorption/desorption experiments on LX60 columns were conducted to obtain the optimal parameters. After one run treatment with LX60, the content of crocin-1 in gardenia yellow reached 29.6%, while geniposide in another fraction reached 83.4%. An extract of crocins was obtained from gardenia yellow in a second-stage separation using RP medium-pressure LC, with its color value to be 756 and the content of crocin-1 reaching 60.8%. The separation process was highly efficient, low cost, and compact, which may be informative for purifications of other natural products from complex plant extracts.
栀子果实含有有价值的天然食用色素，包括藏红花酸（栀子黄）和京尼平苷。本研究采用大孔树脂和反相色谱法从栀子果实中同时富集藏红花酸和京尼平苷。评价了八种不同类型大孔树脂的性能。静态吸附/解吸实验表明，LX60 具有最佳的分离能力。进一步在 LX60 柱上进行动态吸附/解吸实验，以获得最佳参数。经过一次 LX60 处理，栀子黄中藏红花-1 的含量达到 29.6%，而另一部分的京尼平苷含量达到 83.4%。栀子黄中的藏红花酸提取物在使用反相中压 LC 的第二阶段分离中获得，其色值为 756，藏红花-1 的含量达到 60.8%。分离过程高效、低成本、紧凑，可为从复杂植物提取物中纯化其他天然产物提供信息。
