栀子果实中的黄色素：结构鉴定及通过诱导凋亡对HepG2细胞进行细胞毒性活性评估。
Yellow pigment from gardenia fruit: structural identification and evaluation of cytotoxic activity in HepG2 cells by induction of apoptosis.
作者信息
Tang Liqin, Liu Haocheng, Fu Manqin, Xu Yujuan, Wen Jing, Wu Jijun, Yu Yuanshan, Lin Xian, Li Lu, Bu Zhibin, Yang Wanyuan
机构信息
Sericultural & Agri-Food Research Institute Guangdong Academy of Agricultural Sciences, Key Laboratory of Functional Foods, Ministry of Agriculture, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou, 510610 Guangdong China.
College of Agronomy, Jiangxi Agricultural University, Nanchang, 330045 Jiangxi China.
出版信息
Food Sci Biotechnol. 2022 Jul 25;31(11):1389-1399. doi: 10.1007/s10068-022-01133-9. eCollection 2022 Oct.
UNLABELLED
The preparation process of yellow pigment (YP) from gardenia () fruit was investigated, and the main components of YP were characterized by liquid chromatography-time of flight-mass spectrometer/mass spectrometer (LC-TOF-MS/MS). Furthermore, cytotoxic activity in HepG2 cells by induction of apoptosis was also evaluated. The preparation results indicated that the color value of YP was 498.34, which was 8.6 times higher than crude YP. Fifteen compounds in YP were identified, and crocins were the predominant compounds. The cell experiment results showed that YP inhibited the proliferation of HepG2 cells in a time- and dose-dependent manner. Moreover, YP also inhibited HepG2 cells in G2/M stage, increased the level of intracellular reactive oxygen species (ROS), and enhanced cell apoptosis. Real-time quantitative polymerase chain reaction (RT-PCR) analysis revealed the up-regulation of caspase-3, 8, 9, and bax and down-regulation of bcl-2 in HepG2 cells. Overall, these findings suggested that YP had potential cytotoxic activity in HepG2 cells by induction of apoptosis, which might be beneficial to human health.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s10068-022-01133-9.
未标注
研究了从栀子果实中制备黄色素（YP）的工艺，并采用液相色谱-飞行时间-质谱联用仪/质谱仪（LC-TOF-MS/MS）对YP的主要成分进行了表征。此外，还评估了YP在HepG2细胞中通过诱导凋亡产生的细胞毒性活性。制备结果表明，YP的色价值为498.34，比粗制YP高8.6倍。鉴定出YP中的15种化合物，其中西红花苷类为主要化合物。细胞实验结果表明，YP以时间和剂量依赖性方式抑制HepG2细胞的增殖。此外，YP还使HepG2细胞阻滞于G2/M期，增加细胞内活性氧（ROS）水平，并促进细胞凋亡。实时定量聚合酶链反应（RT-PCR）分析显示，HepG2细胞中caspase-3、8、9和bax上调，bcl-2下调。总体而言，这些发现表明YP通过诱导凋亡在HepG2细胞中具有潜在的细胞毒性活性，这可能对人类健康有益。
补充信息
在线版本包含可在10.1007/s10068-022-01133-9获取的补充材料。
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