Braghirolli Daikelly Iglesias, Steffens Daniela, Quintiliano Kerlin, Acasigua Gerson Arisoly Xavier, Gamba Douglas, Fleck Roland A, Petzhold Cesar L, Pranke Patricia
Hematology and Stem Cell Laboratory, Faculty of Pharmacy, Federal University of Rio Grande do Sul, Porto Alegre, 90610-000, Brazil; Post Graduate Programme in Material Science, Federal University of Rio Grande do Sul, Porto Alegre, 91501-970, Brazil.
J Biomed Mater Res B Appl Biomater. 2014 May;102(4):700-8. doi: 10.1002/jbm.b.33049. Epub 2013 Nov 21.
The sterilization of scaffolds is an essential step for tissue engineering in vitro and, mainly, clinical biomaterial use. However, this process can cause changes in the structure and surface of the scaffolds. Therefore, the objective of this study was to investigate the effect of sterilization by ethanol, ultraviolet radiation (UVR) or antimicrobial solution (AMS) on poly(lactide-co-glycolide) (PLGA) scaffolds produced by the electrospinning technique. The properties of nanofibers and the cellular adhesion of mesenchymal stem cells to the scaffolds were analyzed after the treatments. All methods generated sterile scaffolds but showed some kind of damage to the scaffolds. Ethanol and AMS caused changes in the morphology and scaffold dimensions, which were not observed when using the UVR method. However, UVR caused a greater reduction in polymeric molecular weight, which increased proportionally with exposure time of treatment. Nanofibers sterilized with AMS for 1 h and 2 h showed greater cellular adhesion than the other methods, demonstrating their potential as a method for sterilizing PLGA nanofibers.
支架的灭菌是体外组织工程以及主要是临床生物材料使用的关键步骤。然而，这一过程会导致支架的结构和表面发生变化。因此，本研究的目的是探讨乙醇、紫外线辐射（UVR）或抗菌溶液（AMS）灭菌对通过静电纺丝技术制备的聚（丙交酯-共-乙交酯）（PLGA）支架的影响。处理后分析了纳米纤维的性能以及间充质干细胞与支架的细胞黏附情况。所有方法都能产生无菌支架，但对支架都造成了某种程度的损伤。乙醇和AMS导致了形态和支架尺寸的变化，而使用UVR方法时未观察到这种情况。然而，UVR导致聚合物分子量有更大程度的降低，且这种降低与处理的暴露时间成比例增加。用AMS灭菌1小时和2小时的纳米纤维比其他方法表现出更强的细胞黏附，证明了其作为PLGA纳米纤维灭菌方法的潜力。
