Wang Shangqian, Wang Wei, Xu Yang, Tang Min, Fang Jianzheng, Sun Hongyong, Sun Yangyang, Gu Meijuan, Liu Zhili, Zhang Zhaoxia, Lin Faxi, Wu Ting, Song Ninghong, Wang Zengjun, Zhang Wei, Yin Changjun
State Key Laboratory of Reproductive Medicine, Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, P. R. China.
Proteomics. 2014 Feb;14(2-3):298-310. doi: 10.1002/pmic.201300225.
Human sperm cryopreservation in assisted reproductive technology is the only proven method that enables infertile men to father their own children. However, freezing and thawing reduces spermatozoon motility, viability, and fertilizing ability. An association between dysfunctional spermatozoa due to cryoinjury and protein changes has not been established. We investigated through proteomic analysis the differential protein characteristics between freeze-thawed and fresh sperm samples obtained from nine normozoospermic donors. Twenty-seven proteins differed in abundance between the two groups, and results were verified for four proteins via Western blot and immunofluorescent staining. These proteins are putatively involved in sperm motility, viability, acrosomal integrity, ATP and isocitrate content, mitochondrial membrane potential, capacitation, acrosome reaction, and intracellular calcium concentration. These marked differences suggest that dysfunctional spermatozoon after cryopreservation may be due to protein degradation and protein phosphorylation.
辅助生殖技术中的人类精子冷冻保存是唯一经证实能使不育男性生育自己孩子的方法。然而，冷冻和解冻会降低精子活力、存活率和受精能力。冷冻损伤导致的精子功能障碍与蛋白质变化之间的关联尚未确立。我们通过蛋白质组学分析，研究了从9名正常精子捐献者获得的冻融精子样本和新鲜精子样本之间的差异蛋白质特征。两组之间有27种蛋白质丰度不同，通过蛋白质免疫印迹和免疫荧光染色对4种蛋白质的结果进行了验证。这些蛋白质可能参与精子活力、存活率、顶体完整性、ATP和异柠檬酸含量、线粒体膜电位、获能、顶体反应以及细胞内钙浓度。这些显著差异表明，冷冻保存后精子功能障碍可能是由于蛋白质降解和蛋白质磷酸化。
