• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

810nm 近红外光生物调节疗法治疗人类弱精症的疗效。

The power of 810 nm near-infrared photobiomodulation therapy for human asthenozoospermia.

机构信息

SS Physiopathology of Human Reproduction, IRCCS Ospedale Policlinico San Martino, Genova, Italy.

Experimental Medicine Department, University of Genova, Genova, Italy.

出版信息

Sci Rep. 2024 Nov 5;14(1):26819. doi: 10.1038/s41598-024-77823-7.

DOI:10.1038/s41598-024-77823-7
PMID:39501019
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11538380/
Abstract

Sperm motility is a crucial factor in male fertility. Photobiomodulation (PBM) has been reported to increase sperm motility, but a consistent approach suitable for identifying standardizable protocols is lacking. We collected asthenozoospermic (n = 70) and normozoospermic (n = 20) semen. The asthenozoospermic samples were irradiated with an 810 nm diode laser, in continuous wave mode, at 0.25 W, 0.5 W, 1 W and 2 W for 60 s on a circular area of 1 cmthrough a novel handpiece with an innovative flat-top profile. Sperm motility was assessed immediately, after 30 and 60 min. A sample size calculator, unpaired t-test and one-way ANOVA with post-hoc Tukey HSD tests were used for statistics. One and 2 W were the most effective outputs in increasing progressive motility compared to control (p < 0.001). The maximum effect was immediately after 1 W-PBM (p < 0.001) and decreased after 60 min (p < 0.001). Time physiologically decreased vitality (p < 0.001), but less in the 1 W-PBM samples (p < 0.05). 1 W-PBM did not affect chromatin condensation. Asthenozoospermic samples displayed an impairment of 80% in oxygen consumption and ATP production and a slight inefficiency of oxidative phosphorylation compared to normozoospermic samples (p < 0.001). 1 W-PBM partially restored the functionality of aerobic metabolism (p < 0.001) by recovery of oxidative phosphorylation efficiency. PBM did not affect lactate dehydrogenase (glycolysis pathway). No irradiated samples increased accumulated malondialdehyde, a marker of lipidic peroxidation. In conclusion, PBM improves progressive motility in asthenozoospermia through increased mitochondrial energetic metabolism without harmful oxidative stress.

摘要

精子活力是男性生育能力的一个关键因素。已经有报道称光生物调节（PBM）可以提高精子活力，但缺乏一种适用于识别标准化方案的一致方法。我们收集了弱精症（n=70）和正常精子（n=20）的精液。弱精症样本采用 810nm 二极管激光进行照射，连续波模式，在 1cm 的圆形区域上以 0.25W、0.5W、1W 和 2W 的功率照射 60s，使用带有创新平顶轮廓的新型手柄。立即、30 分钟和 60 分钟后评估精子活力。使用样本量计算器、未配对 t 检验和单向方差分析（事后 Tukey HSD 检验）进行统计学分析。与对照组相比，1W 和 2W 是提高前向运动精子活力最有效的输出（p<0.001）。最大效果出现在 1W-PBM 后立即（p<0.001），60 分钟后下降（p<0.001）。时间上生理上降低了活力（p<0.001），但在 1W-PBM 样本中减少较少（p<0.05）。1W-PBM 不影响染色质凝聚。与正常精子样本相比，弱精症样本的耗氧量和 ATP 生成减少了 80%，氧化磷酸化效率略有降低（p<0.001）。1W-PBM 通过恢复氧化磷酸化效率，部分恢复了有氧代谢的功能（p<0.001）。PBM 不影响乳酸脱氢酶（糖酵解途径）。没有照射的样本增加了丙二醛的积累，丙二醛是脂质过氧化的标志物。总之，PBM 通过增加线粒体能量代谢来改善弱精症中的前向运动精子活力，而不会产生有害的氧化应激。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/836d/11538380/faae682e801e/41598_2024_77823_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/836d/11538380/1e7c59a8d581/41598_2024_77823_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/836d/11538380/a4305f83afec/41598_2024_77823_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/836d/11538380/ec7117c6c7e9/41598_2024_77823_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/836d/11538380/fb4a5c2a07c7/41598_2024_77823_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/836d/11538380/1fd78d3cdfed/41598_2024_77823_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/836d/11538380/21c45b17ebb7/41598_2024_77823_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/836d/11538380/2981efb7a07b/41598_2024_77823_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/836d/11538380/02f3bf42232e/41598_2024_77823_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/836d/11538380/faae682e801e/41598_2024_77823_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/836d/11538380/1e7c59a8d581/41598_2024_77823_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/836d/11538380/a4305f83afec/41598_2024_77823_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/836d/11538380/ec7117c6c7e9/41598_2024_77823_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/836d/11538380/fb4a5c2a07c7/41598_2024_77823_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/836d/11538380/1fd78d3cdfed/41598_2024_77823_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/836d/11538380/21c45b17ebb7/41598_2024_77823_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/836d/11538380/2981efb7a07b/41598_2024_77823_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/836d/11538380/02f3bf42232e/41598_2024_77823_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/836d/11538380/faae682e801e/41598_2024_77823_Fig9_HTML.jpg

相似文献

1
The power of 810 nm near-infrared photobiomodulation therapy for human asthenozoospermia.810nm 近红外光生物调节疗法治疗人类弱精症的疗效。
Sci Rep. 2024 Nov 5;14(1):26819. doi: 10.1038/s41598-024-77823-7.
2
Effects of Pulsed-Wave Photobiomodulation Therapy on Human Spermatozoa.脉冲波光生物调节疗法对人类精子的影响。
Lasers Surg Med. 2022 Apr;54(4):540-553. doi: 10.1002/lsm.23399. Epub 2021 Apr 1.
3
Photobiomodulation with 810 nm Wavelengths Improves Human Sperms' Motility and Viability .810nm 波长的光生物调节可提高人类精子的活力和生存能力。
Photobiomodul Photomed Laser Surg. 2020 Apr;38(4):222-231. doi: 10.1089/photob.2019.4773.
4
Photobiomodulation with light-emitting diodes improves sperm motility in men with asthenozoospermia.使用发光二极管进行光生物调节可改善弱精子症男性的精子活力。
Lasers Med Sci. 2015 Jan;30(1):235-40. doi: 10.1007/s10103-014-1653-x. Epub 2014 Sep 10.
5
TAT-peroxiredoxin 2 Fusion Protein Supplementation Improves Sperm Motility and DNA Integrity in Sperm Samples from Asthenozoospermic Men.TAT-过氧化物还原酶2融合蛋白补充剂可改善弱精子症男性精子样本的精子活力和DNA完整性。
J Urol. 2016 Mar;195(3):706-12. doi: 10.1016/j.juro.2015.11.019. Epub 2015 Nov 14.
6
Effect of 830-nm diode laser irradiation on human sperm motility.830纳米二极管激光照射对人类精子活力的影响。
Lasers Med Sci. 2014 Jan;29(1):97-104. doi: 10.1007/s10103-013-1276-7. Epub 2013 Feb 14.
7
High-coverage targeted lipidomics revealed dramatic lipid compositional changes in asthenozoospermic spermatozoa and inverse correlation of ganglioside GM3 with sperm motility.高覆盖靶向脂质组学揭示了弱精子症精子中脂质组成的显著变化，以及神经节苷脂 GM3 与精子运动能力呈负相关。
Reprod Biol Endocrinol. 2021 Jul 7;19(1):105. doi: 10.1186/s12958-021-00792-3.
8
Low human sperm motility coexists with sperm nuclear DNA damage and oxidative stress in semen.人类精子的低活力与精液中精子核 DNA 损伤和氧化应激并存。
Andrology. 2024 Jul;12(5):1154-1169. doi: 10.1111/andr.13556. Epub 2023 Nov 28.
9
Peroxisome Proliferator-Activated Receptors (PPARs) levels in spermatozoa of normozoospermic and asthenozoospermic men.正常精子症和弱精子症男性精子中过氧化物酶体增殖物激活受体（PPARs）水平
Syst Biol Reprod Med. 2019 Dec;65(6):409-419. doi: 10.1080/19396368.2019.1677801. Epub 2019 Nov 1.
10
NMR spectroscopy of live human asthenozoospermic and normozoospermic sperm metabolism.活人体弱精子症和正常精子代谢的 NMR 光谱分析。
Reprod Fertil. 2022 Mar 21;3(2):77-89. doi: 10.1530/RAF-21-0101. eCollection 2022 Apr 1.

引用本文的文献

1
Efficacy and Safety of Visible and Near-Infrared Photobiomodulation Therapy on Astenospermic Human Sperm: Wavelength-Dependent Regulation of Nitric Oxide Levels and Mitochondrial Energetics.可见光和近红外光生物调节疗法对少精子症患者精子的疗效与安全性：一氧化氮水平和线粒体能量代谢的波长依赖性调节
Biology (Basel). 2025 May 1;14(5):491. doi: 10.3390/biology14050491.

本文引用的文献

1
Photobiomodulation on isolated mitochondria at 810 nm: first results on the efficiency of the energy conversion process.810nm 光生物调节对分离线粒体的影响：能量转换过程效率的初步研究结果。
Sci Rep. 2024 May 14;14(1):11060. doi: 10.1038/s41598-024-61740-w.
2
Assessing the Effects of Curcumin and 450 nm Photodynamic Therapy on Oxidative Metabolism and Cell Cycle in Head and Neck Squamous Cell Carcinoma: An In Vitro Study.评估姜黄素和450纳米光动力疗法对头颈部鳞状细胞癌氧化代谢和细胞周期的影响：一项体外研究
Cancers (Basel). 2024 Apr 24;16(9):1642. doi: 10.3390/cancers16091642.
3
Improving human sperm motility via red and near-infrared laser irradiation: in-vitro study.
经红/近红外激光辐照改善人精子活力：体外研究。
Photochem Photobiol Sci. 2024 Feb;23(2):377-385. doi: 10.1007/s43630-023-00525-y. Epub 2024 Jan 27.
4
The Potential of Theophylline and Pentoxifylline in Sperm Optimization and Its Intracytoplasmic Sperm Injection Outcomes.茶碱和己酮可可碱在精子优化及其卵胞浆内单精子注射结局中的潜力
Cureus. 2023 Nov 2;15(11):e48192. doi: 10.7759/cureus.48192. eCollection 2023 Nov.
5
Therapeutic Effects of Low-Level Laser on Male Infertility: A Systematic Review.低强度激光对男性不育症的治疗效果：一项系统评价
J Lasers Med Sci. 2023 Sep 25;14:e36. doi: 10.34172/jlms.2023.36. eCollection 2023.
6
Applications of laser technology in the manipulation of human spermatozoa.激光技术在人类精子操作中的应用。
Reprod Biol Endocrinol. 2023 Oct 21;21(1):93. doi: 10.1186/s12958-023-01148-9.
7
Effects of Deacetylase Inhibition on the Activation of the Antioxidant Response and Aerobic Metabolism in Cellular Models of Fanconi Anemia.去乙酰化酶抑制对范科尼贫血细胞模型中抗氧化反应激活及有氧代谢的影响
Antioxidants (Basel). 2023 May 15;12(5):1100. doi: 10.3390/antiox12051100.
8
Photobiomodulation therapy reverses spermatogenesis arrest in hyperthermia-induced azoospermia mouse model.光生物调节疗法可逆转热诱导无精子症小鼠模型中的精子发生停滞。
Lasers Med Sci. 2023 Apr 27;38(1):114. doi: 10.1007/s10103-023-03780-8.
9
Preclinical and Clinical Applications of Photobiomodulation Therapy in Sperm Motility: A Narrative Review.光生物调节疗法在精子活力方面的临床前和临床应用：一项叙述性综述。
J Lasers Med Sci. 2022 Dec 24;13:e75. doi: 10.34172/jlms.2022.75. eCollection 2022.
10
Temporal trends in sperm count: a systematic review and meta-regression analysis of samples collected globally in the 20th and 21st centuries.精子数量的时间趋势：对 20 世纪和 21 世纪全球采集样本的系统回顾和荟萃回归分析。
Hum Reprod Update. 2023 Mar 1;29(2):157-176. doi: 10.1093/humupd/dmac035.