IVIRMA Global Research Alliance, IVI Foundation, Instituto de Investigación Sanitaria La Fe, Avenida Fernando Abril Martorell, 106-Torre A, Planta 1ª, 46026 Valencia, Spain.
VIDA RECOLETAS Seville, Calle Américo Vespucio, 19, 41092 Seville, Spain.
Int J Mol Sci. 2024 Jul 10;25(14):7582. doi: 10.3390/ijms25147582.
Capacitation involves tyrosine phosphorylation (TP) as a key marker. Lifestyle-related factors, such as obesity and smoking, are recognized for their adverse effects on semen quality and male fertility, yet the underlying mechanisms, including their potential impact on TP, remain unclear. Moreover, the effect of sperm cryopreservation on TP at the human sperm population level is unexplored. Flow cytometry analysis of global TP was performed on pre-capacitated, post-capacitated and 1- and 3-hours' incubated fresh and frozen-thawed samples from sperm donors ( = 40). Neither being overweight nor smoking (or both) significantly affected the percentage of sperm showing TP. However, elevated BMI and smoking intensity correlated with heightened basal TP levels (r = 0.226, = 0.003) and heightened increase in TP after 3 h of incubation (r = 0.185, = 0.017), respectively. Cryopreservation resulted in increased global TP levels after capacitation but not immediately after thawing. Nonetheless, most donors' thawed samples showed increased TP levels before and after capacitation as well as after incubation. Additionally, phosphorylation patterns in fresh and frozen-thawed samples were similar, indicating consistent sample response to capacitation stimuli despite differences in TP levels. Overall, this study sheds light on the potential impacts of lifestyle factors and cryopreservation on the dynamics of global TP levels during capacitation.
获能涉及酪氨酸磷酸化（TP）作为一个关键标记。生活方式相关因素，如肥胖和吸烟，因其对精液质量和男性生育力的不利影响而被公认，但潜在的机制，包括它们对 TP 的潜在影响，仍不清楚。此外，精子冷冻保存对人类精子群体水平上的 TP 的影响仍未被探索。对供精者的预获能、获能后以及 1 小时和 3 小时孵育的新鲜和冷冻解冻样本进行了整体 TP 的流式细胞术分析（n = 40）。超重或吸烟（或两者）均不会显著影响显示 TP 的精子的百分比。然而，BMI 升高和吸烟强度与基础 TP 水平的升高（r = 0.226，p = 0.003）和 3 小时孵育后 TP 的升高相关（r = 0.185，p = 0.017）。获能后冷冻保存会导致 TP 水平升高，但解冻后不会立即升高。尽管如此，大多数供体的解冻样本在获能前后以及孵育后显示出 TP 水平升高。此外，新鲜和冷冻解冻样本中的磷酸化模式相似，表明尽管 TP 水平存在差异，但样本对获能刺激的反应一致。总的来说，这项研究揭示了生活方式因素和冷冻保存对获能过程中整体 TP 水平动态的潜在影响。
