National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), College of Life Science and Technology, and Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan 430070, China.
Proc Natl Acad Sci U S A. 2013 Dec 10;110(50):20320-5. doi: 10.1073/pnas.1319681110. Epub 2013 Nov 20.
Plant metabolites are crucial for both plant life and human nutrition. Despite recent advance in metabolomics, the genetic control of plant metabolome remains largely unknown. Here, we performed a genetic analysis of the rice metabolome that provided over 2,800 highly resolved metabolic quantitative trait loci for 900 metabolites. Distinct and overlapping accumulation patterns of metabolites were observed and complex genetic regulation of metabolism was revealed in two different tissues. We associated 24 candidate genes to various metabolic quantitative trait loci by data mining, including ones regulating important morphological traits and biological processes. The corresponding pathways were reconstructed by updating in vivo functions of previously identified and newly assigned genes. This study demonstrated a powerful tool and provided a vast amount of high-quality data for understanding the plasticity of plant metabolome, which may help bridge the gap between the genome and phenome.
植物代谢物对植物的生命和人类的营养都至关重要。尽管代谢组学最近取得了进展，但植物代谢组的遗传控制在很大程度上仍然未知。在这里，我们对水稻代谢组进行了遗传分析，为 900 种代谢物提供了超过 2800 个高度解析的代谢数量性状位点。在两种不同的组织中观察到了代谢物的不同和重叠的积累模式，并揭示了复杂的代谢遗传调控。我们通过数据挖掘将 24 个候选基因与各种代谢数量性状位点相关联，包括调节重要形态特征和生物过程的基因。通过更新先前鉴定和新分配基因的体内功能，重建了相应的途径。这项研究展示了一种强大的工具，并提供了大量高质量的数据，有助于理解植物代谢组的可塑性，这可能有助于弥合基因组和表型之间的差距。
