冲刺间歇训练诱导大鼠骨骼肌热休克蛋白 72。
Sprint-interval training induces heat shock protein 72 in rat skeletal muscles.
机构信息
Department of Exercise Physiology, School of Health and Sports Science, Juntendo University , Inba, Japan.
出版信息
J Sports Sci Med. 2006 Jun 1;5(2):194-201. eCollection 2006.
Previous studies have demonstrated that endurance exercise training increases the level of heat shock proteins (HSPs) in skeletal muscles. However, little attention has been drawn to the effects of high intensity-short duration exercise, or sprint- interval training (SIT) on HSP72 level in rat skeletal muscles. This study performed to test the hypothesis that the SIT would induce the HSP72 in fast and slow skeletal muscles of rats. Young male Wistar rats (8 weeks old) were randomly assigned to a control (CON) or a SIT group (n = 8/group). Animals in the SIT group were trained (1 min/sprint, 610 sets/day and 56 days/week) on a treadmill for 9 weeks. After the training period, HSP72 levels in the plantaris (fast) and soleus (slow) muscles were analyzed by Western blotting method. Enzyme activities (hexokinase, phosphofructokinase and citrate synthase) and histochemical properties (muscle fiber type compositions and cross sectional area) in both muscles were also determined. The SIT resulted in significantly (p < 0.05) higher levels of HSP72 in both the plantaris and soleus muscles compared to the CON group, with the plantaris producing a greater HSP72 increase than the soleus (plantaris; 550 ± 116%, soleus; 26 ± 8%, p < 0.05). Further, there were bioenergetic improvements, fast-to-slow shift of muscle fiber composition and hypertrophy in the type IIA fiber only in the plantaris muscle. These findings indicate that the SIT program increases HSP72 level of the rat hindlimb muscles, and the SIT-induced accumulation of HSP72 differs between fast and slow muscles. Key PointsThere is no study about the effects of high intensity but short duration exercise, or sprint-interval training (SIT) on heat shock protein 72 (HSP72) level in skeletal muscles.The SIT program (≤ 10 min·day(-1)) accumulated HSP72 in rat skeletal muscles.The SIT-induced accumulation of HSP72 in the plantaris (fast) muscle was drastic compared to the soleus (slow) muscle and accompanied with the improvements of enzyme activities, fast-to-slow shift within fast muscle fiber type and muscle hypertrophy.
先前的研究已经表明，耐力运动训练会增加骨骼肌中的热休克蛋白（HSPs）水平。然而，高强度短时间运动，或冲刺间歇训练（SIT）对大鼠骨骼肌中 HSP72 水平的影响却很少受到关注。本研究旨在验证 SIT 会诱导大鼠快肌和慢肌中 HSP72 的假设。将年轻雄性 Wistar 大鼠（8 周龄）随机分为对照组（CON）或 SIT 组（n = 8/组）。SIT 组的动物在跑步机上进行训练（1 分钟/冲刺，610 组/天，56 天/周），共 9 周。训练结束后，通过 Western blot 法分析比目鱼肌（快肌）和比目鱼肌（慢肌）中的 HSP72 水平。还测定了两肌肉的酶活性（己糖激酶、磷酸果糖激酶和柠檬酸合酶）和组织化学特性（肌纤维类型组成和横截面积）。与 CON 组相比，SIT 组的比目鱼肌和比目鱼肌中的 HSP72 水平均显著升高（p < 0.05），比目鱼肌中的 HSP72 升高幅度大于比目鱼肌（比目鱼肌：550 ± 116%，比目鱼肌：26 ± 8%，p < 0.05）。此外，只有比目鱼肌中的 IIA 型纤维出现了生物能学改善、快肌向慢肌纤维组成的转变以及肥大。这些发现表明，SIT 方案会增加大鼠后肢肌肉的 HSP72 水平，并且 SIT 诱导的 HSP72 在快肌和慢肌之间的积累存在差异。关键点：目前尚无关于高强度但短时间运动，或冲刺间歇训练（SIT）对骨骼肌中热休克蛋白 72（HSP72）水平影响的研究。SIT 方案（≤ 10 分钟·天（-1））会使大鼠骨骼肌中 HSP72 积累。与比目鱼肌（慢肌）相比，SIT 诱导的比目鱼肌（快肌）中 HSP72 的积累幅度较大，同时还伴有酶活性的提高、快肌纤维类型内的快肌向慢肌转变以及肌肉肥大。
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