Alexandrov Oleg S, Divashuk Mikhail G, Yakovin Nikolay A, Karlov Gennady I
Centre for Molecular Biotechnology, Russian State Agrarian University - Moscow Timiryazev Agricultural Academy, Moscow 127550, Timiryazevskaya Street, 49, Russia.
Comp Cytogenet. 2012 Jul 10;6(3):239-47. doi: 10.3897/CompCytogen.v6i3.3261. eCollection 2012.
Humulus japonicus Siebold et Zucc (Japanese hop) is a dioecious species of the family Cannabaceae. The chromosome number is 2n = 16 = 14 + XX for females and 2n = 17 = 14 + XY1Y2 for male. To date, no fluorescence in situ hybridization (FISH) markers have been established for the identification of Humulus japonicus sex chromosomes. In this paper, we report a method for the mitotic and meiotic sex chromosome differentiation in Humulus japonicus by FISH for HJSR, a high copy subtelomeric repeat. The signal is present in the subtelomeric region of one arm of the X chromosome. We demonstrate that males have two Y chromosomes that differ in FISH signal with the HJSR probe. Indeed, the HJSR probe hybridizes to a subtelomeric region on both arms of chromosome Y1 but not of chromosome Y2. The orientation and position of pseudoautosomal regions (PAR1 and PAR2) were also determined.
啤酒花（Humulus japonicus Siebold et Zucc）是大麻科的一种雌雄异株植物。其染色体数目为雌性2n = 16 = 14 + XX，雄性2n = 17 = 14 + XY1Y2。迄今为止，尚未建立用于鉴定啤酒花性染色体的荧光原位杂交（FISH）标记。在本文中，我们报道了一种通过FISH对啤酒花有丝分裂和减数分裂性染色体进行分化的方法，该方法针对一种高拷贝亚端粒重复序列HJSR。该信号存在于X染色体一条臂的亚端粒区域。我们证明雄性有两条Y染色体，它们与HJSR探针的FISH信号不同。实际上，HJSR探针与Y1染色体两条臂上的亚端粒区域杂交，但不与Y2染色体杂交。同时还确定了拟常染色体区域（PAR1和PAR2）的方向和位置。
