Mezzasalma Marcello, Guarino Fabio Maria, Aprea Gennaro, Crottini Angelica, Odierna Gaetano
Dipartimento di Biologia, Università di Napoli Federico II, Via Cinthia I- 80126 Napoli, Italy.
Comp Cytogenet. 2013 Sep 23;7(3):217-27. doi: 10.3897/CompCytogen.v7i3.5398. eCollection 2013.
A karyological analysis on six Italian populations the slow worm (Anguis veronensis Pollini, 1818) was performed and their genetic differentiation at the mitochondrial 16S rRNA gene fragment from a Spanish sample has been assessed. The Italian populations were karyologically uniform, all showing 2n=44 elements, of which 20 were macrochromosomes and 24 microchromosomes. Comparison with literature data on Central European populations showed a difference on the morphology of the 10(th) chromosome pair: submetacentric in Italian populations and telocentric in the Central European ones. Our analysis showed the presence of a fragile site on chromosomes of this pair, suggesting its propensity for structural rearrangements. Analysis of the 16S rRNA gene fragment showed uniformity among Italian populations (uncorrected genetic distance of 0.4%), and their genetic distinctness from the Spanish individual (uncorrected genetic distance of 4.2%). Our results confirm the existence of two different Anguis fragilis Linnaeus, 1758 lineages, each one characterized by a different cytotype.
对意大利六个地区的慢缺肢蜥（Anguis veronensis Pollini，1818）种群进行了核型分析，并评估了它们与一个西班牙样本的线粒体16S rRNA基因片段的遗传分化。意大利的种群在核型上是一致的，均显示2n = 44条染色体，其中20条为常染色体，24条为微小染色体。与中欧种群的文献数据比较显示，第10对染色体的形态存在差异：意大利种群中为亚中着丝粒染色体，而中欧种群中为端着丝粒染色体。我们的分析表明，这对染色体上存在一个脆性位点，表明其有发生结构重排的倾向。对16S rRNA基因片段的分析显示，意大利种群之间具有一致性（未校正遗传距离为0.4%），且它们与西班牙个体在遗传上存在差异（未校正遗传距离为4.2%）。我们的结果证实了1758年林奈命名的两种不同的脆蛇蜥谱系的存在，每种谱系都具有不同的细胞型特征。
