Istituto sperimentale per la Cerealicoltura, Sezione di Bergamo, Via Stezzano 24, I-24100, Bergamo, Italy.
Theor Appl Genet. 1983 May;65(2):123-8. doi: 10.1007/BF00264879.
This paper describes a new dominant mutation of maize, Mc, which interferes in the endosperm with the synthesis of storage proteins. The mutant is characterized by an opaque phenotype; it reduces the deposition of zein and it increases the level of methionine. The mutation is specifically related to storage protein synthesis since soluble and insoluble carbohydrates are present at normal levels. The main interest of this mutant lies in its synergistic interaction with opaque-2 in repressing zein synthesis. In the double mutant o2Mc the accumulation of zein is reduced to less than 10% of that of the normal endosperm. The control on zein synthesis exerted by the double mutant is at the level of production or stability of translatable zein mRNAs. The double mutant o2Mc germinates well offering the opportunity of using it in biochemical and molecular studies related to storage protein synthesis; the reduced endosperm weight of o2Mc negates its practical utilization in breeding maize for quality.
本文描述了玉米的一个新的显性突变体 Mc，该突变体干扰胚乳中贮藏蛋白的合成。该突变体的特征是不透明表型；它减少了醇溶蛋白的沉积，并增加了蛋氨酸的水平。该突变与贮藏蛋白合成密切相关，因为可溶性和不溶性碳水化合物的水平正常。该突变体的主要兴趣在于其与不透明 2 之间的协同作用，可抑制醇溶蛋白的合成。在双突变体 o2Mc 中，醇溶蛋白的积累减少到正常胚乳的 10%以下。双突变体 o2Mc 对醇溶蛋白合成的控制作用在于可翻译的醇溶蛋白 mRNAs 的产生或稳定性。双突变体 o2Mc 发芽良好，为利用其进行与贮藏蛋白合成有关的生化和分子研究提供了机会；o2Mc 的胚乳重量降低否定了其在玉米品质育种中的实际应用。
