Laboratorio per lo studio delle biosintesi vegetali nelle piante di interesse agrario, Milano, Italy.
Theor Appl Genet. 1978 Nov;52(6):263-7. doi: 10.1007/BF00303503.
The synthesis of at least 15 zein polypeptides is under the control of regulatory gene loci. One of these, Opaque-2 (chromosome 7, position 16) strongly reduces the zein accumulation without modifying the zein molecular components. The linkage relationship between the regulatory gene 02 and the 5 structural loci (Zp1, Zp2, Zp3, Zp6, Zp12) segregating with sample Mendelian ratios have been studied. Zp1, Zp2, Zp3 are closely linked to each other; moreover this gene cluster is located on chromosome 7 at 5.5 cM from the Opaque-2 locus. The structural loci Zp6 and Zp12 are not linked with each other, with the 02 locus or with Zp1, Zp2, Zp3. From our data it follows that the zein structural genes are located in at least three positions on the maize genome. The scattering in the genome of the genes controlled by the Opaque-2 locus suggests a transacting role for this regulatory element.
至少有 15 种醇溶蛋白多肽的合成受调控基因座的控制。其中之一是 Opaque-2（第 7 号染色体，16 号位置），它强烈降低醇溶蛋白的积累，而不改变醇溶蛋白的分子成分。研究了与样本孟德尔比例分离的调控基因 02 和 5 个结构基因座（Zp1、Zp2、Zp3、Zp6、Zp12）之间的连锁关系。Zp1、Zp2、Zp3 彼此紧密连锁；此外，这个基因簇位于第 7 号染色体上，距 Opaque-2 基因座 5.5cM。结构基因座 Zp6 和 Zp12 彼此之间不连锁，与 02 基因座或 Zp1、Zp2、Zp3 也不连锁。根据我们的数据，醇溶蛋白结构基因至少位于玉米基因组的三个位置。受 Opaque-2 基因座控制的基因在基因组中的分散表明这个调控元件具有反式作用。
