Department of Entomology, University of Georgia, 30602, Athens, Georgia.
J Chem Ecol. 1990 Dec;16(12):3467-77. doi: 10.1007/BF00982111.
The pedicel, nest paper, and larval silk ofPolistes annularis nests were analyzed by high-resolution solid-state [(13)C]NMR. The pedicel was found to have a high nitrogen content (11%), and the NMR spectra indicated that it is a mixture of carbohydrate and protein. The pedicel protein has an amino acid composition that is very rich in glycine, alanine, serine, and proline (67% of identified residues), similar to that of some insect silks. Solid-state [(13)C]NMR indicated that the nest paper is composed predominantly of cellulose. Silk, spun by matureP. annularis larvae, was shown by [(13)C] NMR and amino acid analysis to be a protein very high in serine and alanine (53%), but the amino acid composition is distinct from that of the pedicel protein.
利用高分辨率固态 [(13)C]NMR 对 Polistes annularis 巢的花梗、巢纸和幼虫丝进行了分析。结果表明,花梗的含氮量较高(11%),其 NMR 谱表明它是碳水化合物和蛋白质的混合物。花梗蛋白的氨基酸组成非常丰富,富含甘氨酸、丙氨酸、丝氨酸和脯氨酸(鉴定出的残基的 67%),类似于某些昆虫丝。固态 [(13)C]NMR 表明,巢纸主要由纤维素组成。利用 [(13)C]NMR 和氨基酸分析表明,由成熟的 P. annularis 幼虫吐出的丝是一种丝氨酸和丙氨酸含量非常高的蛋白质(53%),但其氨基酸组成与花梗蛋白明显不同。