Akerlund A S, Hanson L A, Ahlstedt S, Carlsson B
Scand J Immunol. 1977;6(12):1275-82. doi: 10.1111/j.1365-3083.1977.tb00366.x.
A method to quantitate specifically secretory IgA (SIgA) has been developed using the enzyme-linked immunosorbent assay. The IgA in the test sample was adsorbed to anti-alpha antibodies attached to plastic tubes via a cost of IgA myeloma protein. The reacted SIgA was determined using anti-secretory component antiserum conjugated with alkaline phosphatase. The technique permitted quantitation of secretory IgA in biological fluids like milk, urine, and saliva with a reproducibility of +/-7%, down to 0.03 mg/l. In contrast to earlier techniques, the presence of up to 157% of serum IgA without secretory component (SC) and free SC did not disturb the measurements of SIgA. Furthermore, variations in pH and osmolarity, within biological ranges in secretions, did not influence the estimations.
一种使用酶联免疫吸附测定法定量特异性分泌型 IgA（SIgA）的方法已经开发出来。测试样品中的 IgA 通过 IgA 骨髓瘤蛋白的载体吸附到附着在塑料管上的抗α抗体上。使用与碱性磷酸酶偶联的抗分泌成分抗血清测定反应的 SIgA。该技术能够定量牛奶、尿液和唾液等生物体液中的分泌型 IgA，重现性为±7%，最低检测限为 0.03 mg/l。与早期技术不同的是，高达 157%的无分泌成分（SC）血清 IgA 和游离 SC 的存在并不干扰 SIgA 的测量。此外，分泌物生物学范围内的 pH 和渗透压变化不影响测定结果。
