Sack D A, Neogi P K, Alam M K
Infect Immun. 1980 Jul;29(1):281-3. doi: 10.1128/iai.29.1.281-283.1980.
We have developed an enzyme-linked immunosorbent assay for quantitating human immunoglobulin A in human secretions and serum. The procedure uses Immunobeads as the solid phase, has a sensitivity of 10 ng/ml, and can be completed in 1 day. Since there is no diffusion through agar, the assay does not require a correction factor when measuring secretory immunoglobulin A. This method should be useful in studies of local mucosal immunity in humans, especially when a very sensitive assay is needed.
我们已经开发出一种酶联免疫吸附测定法，用于定量检测人体分泌物和血清中的人免疫球蛋白A。该方法使用免疫珠作为固相，灵敏度为10 ng/ml，且可在1天内完成。由于不存在通过琼脂的扩散，在测量分泌型免疫球蛋白A时该测定法不需要校正因子。这种方法在人类局部黏膜免疫研究中应该会很有用，特别是在需要非常灵敏的测定法时。
