Department of Biology, Vanderbilt University, Nashville, Tennessee 37235.
Plant Physiol. 1979 Aug;64(2):327-31. doi: 10.1104/pp.64.2.327.
A phytochrome radioimmunoassay with a detection limit of about 2 nanograms has been developed. The radioimmunoassay does not suffer from the potential drawbacks of the commonly used spectral assay and requires less than 1 microliter of crude extract from dark-grown plants for quantitation of phytochrome. Measurement of phytochrome in crude extracts by radioimmunoassay gives values about 25% greater than those obtained by spectral assay. The amount of phytochrome detected in crude extracts of light-grown oats by radioimmunoassay is approximately 1% of that detected in comparable extracts from dark-grown oats. General interference by crude plant extracts with radioimmunoassays was also observed and corrected for.
已经开发出一种检测限约为 2 纳克的光敏色素放射免疫测定法。该放射免疫测定法不受常用光谱测定法的潜在缺陷的影响，并且对于定量测定植物色素，只需要来自黑暗生长植物的粗提取物的小于 1 微升。通过放射免疫测定法测量粗提取物中的植物色素给出的值比通过光谱测定法获得的值约大 25％。通过放射免疫测定法从光生长燕麦的粗提取物中检测到的植物色素的量约为从黑暗生长燕麦的可比提取物中检测到的植物色素的 1％。还观察到并校正了粗植物提取物对放射免疫测定法的一般干扰。
