从 Phycomyces 孢子梗中分析微粒体黄素蛋白：候选蓝光光受体。

Analysis of microsomal flavoproteins from Phycomyces sporangiophores: Candidates for the blue-light photoreceptor.

机构信息

Department of Physics, Syracuse University, 13210, Syracuse, New York, USA.

出版信息

Planta. 1985 Apr;163(4):506-16. doi: 10.1007/BF00392708.

Abstract

To help identify components of the blue-light photoreceptor system for phototropism in Phycomyces blakesleeanus Bgff., proteins from a microsomal fraction obtained from synchronous sporangiophores were studied. By two-dimensional gel electrophoresis, two proteins (FP1, FP2) with covalently bound flavins were found. FP1 has a molecular weight of 71 000 and an isoelectric point of 6.6; FP2 has a molecular weight of 59 000 and an isoelectric point of 6.5. These flavoproteins were purified by column chromatography and gel filtration while assaying for flavins by fluorescence. The relative concentrations of FP1 and FP2 were affected by light applied during growth. These flavoproteins are likely components of the blue-light photoreceptor complex mediating phototropism in Phycomyces.

摘要

为了帮助鉴定出泡囊藻（Phycomyces blakesleeanus Bgff.）光向性中的蓝光光受体系统的组成成分，我们研究了从同步游动孢子囊中获得的微粒体部分的蛋白质。通过二维凝胶电泳，发现了两种带有共价结合黄素的蛋白质（FP1、FP2）。FP1 的分子量为 71000，等电点为 6.6；FP2 的分子量为 59000，等电点为 6.5。这些黄素蛋白通过柱层析和凝胶过滤进行纯化，同时通过荧光法测定黄素。FP1 和 FP2 的相对浓度受生长过程中光照的影响。这些黄素蛋白可能是介导泡囊藻光向性的蓝光光受体复合物的组成部分。