Astier-Gin T, Galiay M, Legrand E, Moynet D, Rebeyrotte N, Artus A, Guillemain B, Duplan J F
Leuk Res. 1986;10(7):809-17. doi: 10.1016/0145-2126(86)90302-4.
The role of retroviruses in murine radioleukemogenesis was reinvestigated using a protocol associating the injection of a non-pathogenic retrovirus (T1223/B virus) and a subleukemogenic dose of X-radiation (2 X 1.75 Gy). Using the Southern blotting technique we studied MuLV proviral organization and RNA expression in thymic lymphomas induced by the combined effect of virus and irradiation or irradiation alone. A recombinant provirus was detected in the chromosomal DNA of every tumor induced by associating virus and radiation whereas it was unconstantly found in radio-induced tumors. In every instance, the provirus was not integrated at a common site. No relationship was observed between viral RNA expression and tumor induction. Trisomy 15 was observed in all metaphases irrespective of the protocol of tumor induction. The G-banding technique revealed an extra-band in several thymic lymphomas induced by irradiation and T1223/B virus injection.
使用一种将注射非致病性逆转录病毒（T1223/B病毒）与亚致白血病剂量的X射线（2×1.75 Gy）相结合的方案，对逆转录病毒在小鼠放射性白血病发生中的作用进行了重新研究。我们使用Southern印迹技术，研究了由病毒和辐射联合作用或单独辐射诱导的胸腺淋巴瘤中莫洛尼鼠白血病病毒（MuLV）前病毒的组织形式和RNA表达。在由病毒和辐射联合诱导的每一个肿瘤的染色体DNA中都检测到了一种重组前病毒，而在辐射诱导的肿瘤中则不常发现。在每种情况下，前病毒都没有整合到一个共同的位点。未观察到病毒RNA表达与肿瘤诱导之间的关系。无论肿瘤诱导方案如何，在所有中期相中都观察到了15号染色体三体。G带技术揭示，在由辐射和注射T1223/B病毒诱导的几个胸腺淋巴瘤中出现了一条额外的带。
