Thomas C Y, Boykin B J, Famulari N G, Coppola M A
J Virol. 1986 May;58(2):314-23. doi: 10.1128/JVI.58.2.314-323.1986.
We determined the phenotype and genotype of murine leukemia viruses associated with the development of spontaneous nonthymic lymphomas in the high-leukemia mouse strain CWD/J. By T1 oligonucleotide fingerprint analysis of the viral RNA, the ecotropic viruses recovered from the spleen or thymus of preleukemic CWD/J mice were found to represent the progeny of the two endogenous ecotropic proviruses present in this strain. Polytropic murine leukemia viruses were produced by tissues from one-half of the leukemic mice, and fresh tumor cells from one of the two animals tested expressed recombinant envelope glycoproteins. The genomic structure of the recombinant viruses resembled those of class II polytropic viruses of NFS X Akv mice and differed from those of class I recombinant viruses that are commonly isolated from other high-leukemia strains such as AKR and HRS. Acquired retroviral sequences with the structural features of class II recombinant proviruses were detected in the DNA from each CWD/J tumor by the Southern blot technique. Finally, the injection of a mixture of CWD/J ecotropic and class II recombinant polytropic viruses into neonatal CWD/J mice accelerated the onset of lymphoma, whereas the endogenous ecotropic virus was inactive in these assays.
我们确定了与高白血病小鼠品系CWD/J中自发性非胸腺淋巴瘤发生相关的鼠白血病病毒的表型和基因型。通过对病毒RNA进行T1寡核苷酸指纹分析，发现从白血病前期CWD/J小鼠的脾脏或胸腺中分离出的嗜亲性病毒代表了该品系中存在的两种内源性嗜亲性前病毒的后代。多嗜性鼠白血病病毒由一半白血病小鼠的组织产生，在两只受试动物之一的新鲜肿瘤细胞中表达了重组包膜糖蛋白。重组病毒的基因组结构类似于NFS×Akv小鼠的II类多嗜性病毒，与通常从其他高白血病品系（如AKR和HRS）中分离出的I类重组病毒不同。通过Southern印迹技术在每个CWD/J肿瘤的DNA中检测到具有II类重组前病毒结构特征的获得性逆转录病毒序列。最后，将CWD/J嗜亲性病毒和II类重组多嗜性病毒的混合物注射到新生CWD/J小鼠中加速了淋巴瘤的发病，而内源性嗜亲性病毒在这些试验中无活性。
