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血小板膜糖蛋白GPIIb/IIIa与多种细胞表达的细胞表面分子之间的免疫关系。

Immunologic relationship between platelet membrane glycoprotein GPIIb/IIIa and cell surface molecules expressed by a variety of cells.

作者信息

Plow E F, Loftus J C, Levin E G, Fair D S, Dixon D, Forsyth J, Ginsberg M H

出版信息

Proc Natl Acad Sci U S A. 1986 Aug;83(16):6002-6. doi: 10.1073/pnas.83.16.6002.

DOI:10.1073/pnas.83.16.6002
PMID:2426709
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC386425/
Abstract

A polyclonal antiserum to platelet membrane glycoprotein GPIIb/IIIa was used to detect antigenically related molecules on a diverse panel of human cells. Umbilical vein endothelial cells, erythroleukemic HEL cells, and diploid fetal lung GM1380 fibroblasts expressed GPIIb/IIIa-related molecules, as judged by immunofluorescence and immunoprecipitation of surface-labeled proteins. The GPIIb and GPIIIa subunits were both present and were of similar molecular weight in these cell types. These molecules were synthetic products of the cells, as shown by immunoprecipitation of intrinsically labeled proteins. Promyeloid U937 cells could be induced by 4 beta-phorbol 12-myristate 13-acetate to synthesize and express GPIIb/IIIa-related molecules on their cell surface. The GPIIb/IIIa-related molecules were not precisely identical in the various cell types, based on slight differences in electrophoretic mobility and their failure to react with monoclonal antibodies specific for each subunit of platelet GPIIb/IIIa. These results suggest the existence of a widely distributed family of GPIIb/IIIa-related molecules. This family of "cytoadhesins" may share a common function in cellular adhesive reactions.

摘要

一种针对血小板膜糖蛋白GPIIb/IIIa的多克隆抗血清被用于检测多种人类细胞上抗原相关分子。通过免疫荧光和表面标记蛋白的免疫沉淀判断，脐静脉内皮细胞、红白血病HEL细胞和二倍体胎儿肺GM1380成纤维细胞表达GPIIb/IIIa相关分子。在这些细胞类型中，GPIIb和GPIIIa亚基均存在且分子量相似。如内在标记蛋白的免疫沉淀所示，这些分子是细胞的合成产物。早幼粒细胞U937细胞可被4β-佛波醇12-肉豆蔻酸酯13-乙酸酯诱导，在其细胞表面合成并表达GPIIb/IIIa相关分子。基于电泳迁移率的细微差异以及它们与血小板GPIIb/IIIa每个亚基特异性单克隆抗体不发生反应，不同细胞类型中的GPIIb/IIIa相关分子并不完全相同。这些结果提示存在一个广泛分布的GPIIb/IIIa相关分子家族。这个“细胞粘附素”家族可能在细胞粘附反应中具有共同功能。

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