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血小板糖蛋白IIb。染色体定位与组织表达。

Platelet glycoprotein IIb. Chromosomal localization and tissue expression.

作者信息

Bray P F, Rosa J P, Johnston G I, Shiu D T, Cook R G, Lau C, Kan Y W, McEver R P, Shuman M A

机构信息

Department of Medicine, University of California, San Francisco 94143.

出版信息

J Clin Invest. 1987 Dec;80(6):1812-7. doi: 10.1172/JCI113277.

DOI:10.1172/JCI113277
PMID:3479442
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC442459/
Abstract

The GPIIb-IIIa complex functions as a receptor for cytoadhesive proteins on the platelet surface. Both GPIIb and GPIIIa are synthesized by a human erythroleukemia (HEL) cell line. We isolated several cDNA clones by screening a HEL cell cDNA library with an oligonucleotide derived from amino acid sequence of GPIIb. Nucleotide and amino acid sequences were determined from 703 bp of one of these clones. Amino acid sequence of purified platelet GPIIb peptides confirmed the identity of the clone. The cDNA encodes the carboxyl terminus of the large (alpha) subunit of GPIIb and all of the smaller (beta) subunit of GPIIb. By hybridizing the cDNA directly to chromosomes separated by dual laser chromosome sorting, the gene for GPIIb was mapped to chromosome 17. Northern blot analysis showed a approximately 3.4-kb GPIIb mRNA in HEL cells. We also compared the amino acid sequences determined from eight additional platelet GPIIb peptides with the derived amino acids from a published HEL cell GPIIb cDNA, and the platelet and HEL cell proteins appear to be the same. Despite previous reports that vascular endothelial cells and monocytes contain GPIIb, no GPIIb mRNA was observed in either type of cell. Thus, GPIIb appears to be specific for the platelet-megakaryocyte membrane and is distinct from the alpha subunits of the adhesion receptors in other normal tissues.

摘要

血小板膜糖蛋白IIb-IIIa复合物作为血小板表面细胞粘附蛋白的受体发挥作用。糖蛋白IIb和糖蛋白IIIa均由人红白血病（HEL）细胞系合成。我们用从糖蛋白IIb氨基酸序列推导而来的寡核苷酸筛选HEL细胞cDNA文库，分离出了几个cDNA克隆。测定了其中一个克隆703bp的核苷酸和氨基酸序列。纯化的血小板糖蛋白IIb肽的氨基酸序列证实了该克隆的一致性。该cDNA编码糖蛋白IIb大亚基（α）的羧基末端以及糖蛋白IIb的所有小亚基（β）。通过将该cDNA直接与经双激光染色体分拣分离的染色体杂交，将糖蛋白IIb基因定位于17号染色体。Northern印迹分析显示HEL细胞中有一条约3.4kb的糖蛋白IIb mRNA。我们还将另外8个血小板糖蛋白IIb肽的氨基酸序列与已发表的HEL细胞糖蛋白IIb cDNA推导的氨基酸进行了比较，血小板和HEL细胞的蛋白似乎是相同的。尽管先前有报道称血管内皮细胞和单核细胞含有糖蛋白IIb，但在这两种细胞类型中均未观察到糖蛋白IIb mRNA。因此，糖蛋白IIb似乎是血小板-巨核细胞膜所特有的，并且与其他正常组织中粘附受体的α亚基不同。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffd2/442459/76dc61d44418/jcinvest00096-0307-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffd2/442459/7fefa506f47b/jcinvest00096-0307-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffd2/442459/f2f24ad10cb2/jcinvest00096-0307-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffd2/442459/af6aed993835/jcinvest00096-0307-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffd2/442459/76dc61d44418/jcinvest00096-0307-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffd2/442459/7fefa506f47b/jcinvest00096-0307-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffd2/442459/f2f24ad10cb2/jcinvest00096-0307-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffd2/442459/af6aed993835/jcinvest00096-0307-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffd2/442459/76dc61d44418/jcinvest00096-0307-d.jpg

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本文引用的文献

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Human blood monocytes and platelets share a cell surface component.人类血液中的单核细胞和血小板共有一种细胞表面成分。
Blood. 1982 Sep;60(3):767-71.
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High-resolution chromosome sorting and DNA spot-blot analysis assign McArdle's syndrome to chromosome 11.高分辨率染色体分选和DNA斑点印迹分析将麦克尔憩室综合征定位于11号染色体。
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Molecular defects in interactions of platelets with the vessel wall.血小板与血管壁相互作用中的分子缺陷。
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Platelet-targeted gene therapy with human factor VIII establishes haemostasis in dogs with haemophilia A.人凝血因子 VIII 靶向血小板基因治疗可在血友病 A 犬中止血。
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The GPIIb/IIIa (integrin alphaIIbbeta3) odyssey: a technology-driven saga of a receptor with twists, turns, and even a bend.血小板糖蛋白IIb/IIIa（整合素αIIbβ3）的历程：一个受体由技术驱动的曲折传奇，甚至还有一个转折。
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The Pl(A2) polymorphism of integrin beta(3) enhances outside-in signaling and adhesive functions.整合素β3的Pl(A2)多态性增强了由外向内信号传导和黏附功能。
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Glanzmann thrombasthenia. Cooperation between sequence variants in cis during splice site selection.血小板无力症。剪接位点选择过程中顺式序列变异之间的协同作用。
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Homologous recombination among three intragene Alu sequences causes an inversion-deletion resulting in the hereditary bleeding disorder Glanzmann thrombasthenia.三个基因内Alu序列之间的同源重组导致倒位缺失，进而引发遗传性出血性疾病——Glanzmann血小板无力症。
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Chromosomal location of the genes encoding the leukocyte adhesion receptors LFA-1, Mac-1 and p150,95. Identification of a gene cluster involved in cell adhesion.编码白细胞黏附受体LFA-1、Mac-1和p150,95的基因的染色体定位。参与细胞黏附的基因簇的鉴定。
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6
Monoclonal antibodies specific for platelet glycoproteins react with human monocytes.针对血小板糖蛋白的单克隆抗体可与人单核细胞发生反应。
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