Key Laboratory of Cardiovascular Disease and Molecular Intervention, State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China.
CNS Neurosci Ther. 2013 Dec;19(12):917-25. doi: 10.1111/cns.12199. Epub 2013 Nov 4.
Remote ischemic preconditioning protects against ischemic organ damage by giving short periods of subcritical ischemia to a remote organ. We tested the hypothesis that remote ischemic conditioning can attenuate cerebral stroke in a rat middle cerebral artery occlusion (MCAO) model by microparticles (MPs).
MPs were extracted from healthy rats that underwent hindlimb ischemia-reperfusion preconditioning (RIPC), and were transfused into rats that had undergone MCAO without RIPC. The transfusion resulted in an increase in platelet-derived MPs in blood and reduction in infarction area, confirmed by both 2-3-5-triphenyltetrazolium chloride staining and magnetic resonance imaging, albeit to a lesser degree than RIPC itself. Behavioral tests (modified Neurological Severity Score [mNSS]) were calculated to judge the behavioral change. However, no significant difference was observed after MP transfusion in 24 h or the following consecutive 9 days.
RIPC induces an increase in MPs, and platelet-derived MPs may confer at least part of the remote protective effect against cerebral ischemic-reperfusion injury.
通过对远程器官进行短暂的亚临界缺血，远程缺血预处理可防止缺血性器官损伤。我们通过微粒（MPs）测试了这样一个假设，即远程缺血预处理可以减轻大鼠大脑中动脉闭塞（MCAO）模型中的脑卒。
从经历过下肢缺血再灌注预处理（RIPC）的健康大鼠中提取 MPs，并输注到未经历 RIPC 的 MCAO 大鼠中。通过 2-3-5-三苯基氯化四氮唑染色和磁共振成像证实，输注导致血液中血小板衍生的 MPs 增加，梗塞面积减少，但程度低于 RIPC 本身。通过改良神经功能缺损评分（mNSS）计算行为测试来判断行为变化。然而，MP 输注后 24 小时或随后的连续 9 天，观察到的差异没有统计学意义。
RIPC 诱导 MPs 增加，血小板衍生的 MPs 可能至少部分赋予对脑缺血再灌注损伤的远程保护作用。
