Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Pestic Biochem Physiol. 2013 Nov;107(3):360-8. doi: 10.1016/j.pestbp.2013.10.004. Epub 2013 Oct 11.
Based on a Leptinotarsa decemlineata transcriptome dataset and the GenBank sequences, a total of 74 cytochrome P450 monooxygenase genes (Cyps) were identified. These genes fell into CYP2 clan, mitochondrial clan, CYP3 clan and CYP4 clan, and were classified into 19 families and 35 subfamilies according to standard nomenclature. Two new families were discovered in CYP4 clan, and were named CYP412 and CYP413 respectively. Four new families that were recently discovered in Tribolium castaneum, including mitochondrial family CYP353, CYP3 clan families CYP345 and CYP347, and CYP4 clan family CYP350, were also found in L. decemlineata. The phylogenetic trees of CYPs from L. decemlineata and other representative insect species were constructed, and these trees provided evolutionary insight for the genetic distance. Our results facilitate further researches to understand the functions and evolution of L. decemlineata Cyp genes. In order to find cyhalothrin-inducible Cyp genes, the expression levels of Cyps belonging to CYP12, CYP6, CYP9 and CYP4 families were determined by quantitative reverse transcriptase-PCR in cyhalothrin-treated and control fourth-instar larvae. Nine Cyp genes, i.e., Cyp12H2, Cyp6BH2, Cyp6BJ1, Cyp6BQ17, Cyp6EG1, Cyp6EH1, Cyp6EJ1 Cyp4BN13v1 and Cyp4BN15, were highly expressed in cyhalothrin-treated larvae. These CYPs are the candidates that are involved in cyhalothrin detoxification.
基于马铃薯块茎蛾转录组数据集和 GenBank 序列，共鉴定出 74 个细胞色素 P450 单加氧酶基因（Cyps）。这些基因属于 CYP2 族、线粒体族、CYP3 族和 CYP4 族，并根据标准命名法分为 19 个家族和 35 个亚家族。在 CYP4 族中发现了两个新家族，分别命名为 CYP412 和 CYP413。在马铃薯块茎蛾中还发现了四个最近在赤拟谷盗中发现的新家族，包括线粒体家族 CYP353、CYP3 族家族 CYP345 和 CYP347，以及 CYP4 族家族 CYP350。从马铃薯块茎蛾和其他代表性昆虫物种中构建了 CYPs 的系统发育树，这些树为遗传距离提供了进化见解。我们的结果有助于进一步研究了解马铃薯块茎蛾 Cyp 基因的功能和进化。为了寻找氰戊菊酯诱导的 Cyp 基因，通过定量逆转录聚合酶链式反应测定了属于 CYP12、CYP6、CYP9 和 CYP4 家族的 Cyps 在氰戊菊酯处理和对照的第四龄幼虫中的表达水平。在氰戊菊酯处理的幼虫中，9 个 Cyp 基因，即 Cyp12H2、Cyp6BH2、Cyp6BJ1、Cyp6BQ17、Cyp6EG1、Cyp6EH1、Cyp6EJ1 Cyp4BN13v1 和 Cyp4BN15，表达水平较高。这些 CYP 是参与氰戊菊酯解毒的候选基因。
