Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.
Academy of Agricultural Sciences, Southwest University, Chongqing, China.
PLoS One. 2021 Mar 24;16(3):e0248749. doi: 10.1371/journal.pone.0248749. eCollection 2021.
The striped flea beetle, Phyllotreta striolata (Fabricius), damages crops in the Brassicaceae. The genetic data for this pest are insufficient to reveal its insecticide resistance mechanisms or to develop molecular markers for resistance monitoring. We used PacBio Iso-Seq technology to sequence the full-length transcriptome of P. striolata. After isoform sequence clustering and removal of redundant transcripts, a total of 41,293 transcripts were obtained, and 35,640 of these were annotated in the database of gene products. Structure analysis uncovered 4,307 alternative splicing events, and 3,836 sequences were recognized as lncRNAs. Transcripts with the complete coding region of important detoxification enzymes were further classified. There were 57 transcripts of P450s distributed in CYP2, CYP3, CYP4, and Mito CYP clades, 29 transcripts of ESTs from 4 functional groups, 17 transcripts of GSTs classified into 5 families, 51 transcripts of ABCs distributed in 6 families, and 19 transcripts of UGTs. Twenty-five lncRNAs were predicted to be regulators of these detoxification genes. Full-length transcriptome sequencing is an efficient method for molecular study of P. striolata and it is also useful for gene function analysis.
横纹潜叶跳甲，Phyllotreta striolata (Fabricius)，对十字花科作物造成损害。这种害虫的遗传数据不足以揭示其杀虫剂抗性机制，也无法开发用于抗性监测的分子标记。我们使用 PacBio Iso-Seq 技术对 P. striolata 的全长转录组进行测序。经过同工型序列聚类和冗余转录本去除，共获得 41293 条转录本，其中 35640 条在基因产物数据库中得到注释。结构分析揭示了 4307 个可变剪接事件，3836 个序列被识别为 lncRNA。对重要解毒酶的完整编码区的转录本进行了进一步分类。CYP2、CYP3、CYP4 和 Mito CYP 簇中分布着 57 个 P450s 的转录本，4 个功能组的 29 个 ESTs 转录本，5 个家族的 17 个 GSTs 转录本，6 个家族中的 51 个 ABCs 转录本，以及 19 个 UGTs 转录本。25 个 lncRNA 被预测为这些解毒基因的调控因子。全长转录组测序是研究 P. striolata 的分子生物学的有效方法，也有助于基因功能分析。
