Clements Justin, Schoville Sean, Peterson Nathan, Huseth Anders S, Lan Que, Groves Russell L
Department of Entomology, University of Wisconsin-Madison, Madison, WI 53706, USA.
University of Wisconsin-Madison, UW-Health and Clinics, Madison, WI 53792, USA.
Pestic Biochem Physiol. 2017 Jan;135:35-40. doi: 10.1016/j.pestbp.2016.07.001. Epub 2016 Jul 9.
The Colorado potato beetle, Leptinotarsa decemlineata (Say), is a major agricultural pest of potatoes in the Central Sands production region of Wisconsin. Previous studies have shown that populations of L. decemlineata have become resistant to many classes of insecticides, including the neonicotinoid insecticide, imidacloprid. Furthermore, L. decemlineata has multiple mechanisms of resistance to deal with a pesticide insult, including enhanced metabolic detoxification by cytochrome p450s and glutathione S-transferases. With recent advances in the transcriptomic analysis of imidacloprid susceptible and resistant L. decemlineata populations, it is possible to investigate the role of candidate genes involved in imidacloprid resistance. A recently annotated transcriptome analysis of L. decemlineata was obtained from select populations of L. decemlineata collected in the Central Sands potato production region, which revealed a subset of mRNA transcripts constitutively up-regulated in resistant populations. We hypothesize that a portion of the up-regulated transcripts encoding for genes within the resistant populations also encode for pesticide resistance and can be suppressed to re-establish a susceptible phenotype. In this study, a discrete set of three up-regulated targets were selected for RNA interference experiments using a resistant L. decemlineata population. Following the successful suppression of transcripts encoding for a cytochrome p450, a cuticular protein, and a glutathione synthetase protein in a select L. decemlineata population, we observed reductions in measured resistance to imidacloprid that strongly suggest these genes control essential steps in imidacloprid metabolism in these field populations.
科罗拉多马铃薯甲虫，即十星瓢虫（Leptinotarsa decemlineata (Say)），是威斯康星州中部沙地马铃薯产区的主要农业害虫。先前的研究表明，十星瓢虫种群已对包括新烟碱类杀虫剂吡虫啉在内的许多种类杀虫剂产生了抗性。此外，十星瓢虫具有多种抗性机制来应对农药侵害，包括细胞色素P450和谷胱甘肽S-转移酶增强的代谢解毒作用。随着对吡虫啉敏感和抗性十星瓢虫种群转录组分析的最新进展，有可能研究参与吡虫啉抗性的候选基因的作用。最近从在中部沙地马铃薯产区采集的十星瓢虫特定种群中获得了十星瓢虫的注释转录组分析结果，该分析揭示了抗性种群中组成性上调的一部分mRNA转录本。我们假设，抗性种群中上调的一部分转录本所编码的基因也编码农药抗性，并且可以被抑制以重新建立敏感表型。在本研究中，从抗性十星瓢虫种群中选择了一组离散的三个上调靶点进行RNA干扰实验。在选定的十星瓢虫种群中成功抑制了编码细胞色素P450、一种表皮蛋白和一种谷胱甘肽合成酶蛋白的转录本后，我们观察到对吡虫啉的实测抗性降低，这强烈表明这些基因控制了这些田间种群中吡虫啉代谢的关键步骤。
