Delmage J M, Powars D R, Jaynes P K, Allerton S E
Ann Clin Lab Sci. 1986 Jul-Aug;16(4):303-10.
A hyaluronidase-sensitive component of human peritoneal fluid from a patient with Wilms' tumor when injected into rabbits has been shown to suppress the formation of humoral precipitating antibodies to certain major classes of proteins present in the fluid. Furthermore, it has been found that hyaluronic acid, when included with certain test antigens (serum albumin, fetuin) or antigen mixtures (tumor isolates or mixtures of albumin, immunoglobulin G and immunoglobulin M), produces a marked distortion or complete blockage of immunoelectrophoresis precipitin arcs, as well as altered gel chromatography elution profiles. These findings that hyaluronic acid can interfere profoundly with both the elicitation of a complete antibody response and the formation of "normal" patterns of antigen-antibody precipitates in laboratory tests supports the possibility that this polysaccharide may play an immuno-regulatory role by masking potential immunogens. Consideration of the mechanisms for these in vivo and in vitro effects suggests that there may be some common basis in an "excluded volume" property of the hyaluronate, but this does not appear sufficient to explain the complexity and selectivity of the observed phenomena.
已证明，将一名患有肾母细胞瘤患者的人腹膜液中对透明质酸酶敏感的成分注入兔子体内后，可抑制针对该液体中某些主要蛋白质类别的体液沉淀抗体的形成。此外，还发现，当透明质酸与某些测试抗原（血清白蛋白、胎球蛋白）或抗原混合物（肿瘤分离物或白蛋白、免疫球蛋白G和免疫球蛋白M的混合物）一起存在时，会导致免疫电泳沉淀弧明显变形或完全阻断，同时凝胶色谱洗脱图谱也会改变。这些发现表明，透明质酸在实验室测试中可深刻干扰完整抗体反应的引发以及抗原-抗体沉淀“正常”模式的形成，这支持了这种多糖可能通过掩盖潜在免疫原发挥免疫调节作用的可能性。对这些体内和体外效应机制的思考表明，透明质酸盐的“排阻体积”特性可能存在一些共同基础，但这似乎不足以解释所观察到现象的复杂性和选择性。
