Franklin W A, Ringus J C
Lab Invest. 1981 Apr;44(4):375-80.
A basement membrane antigen was prepared from renal cortical basement membranes by partial reduction and alkylation. The antigen was partially purified by gel filtration chromatography. By indirect immunofluorescence, the antigen was found within five Wilms' tumors adjacent to tumor cells. The location and configuration of antigen was similar to that of flocculent electron-dense material observed ultrastructurally. The localization of this antigen provides immunohistochemical evidence for the production of basement membrane components by Wilms' tumors.
通过部分还原和烷基化从肾皮质基底膜制备了一种基底膜抗原。该抗原通过凝胶过滤色谱法进行了部分纯化。通过间接免疫荧光法，在五个与肿瘤细胞相邻的肾母细胞瘤内发现了该抗原。抗原的定位和形态与超微结构观察到的絮状电子致密物质相似。这种抗原的定位为肾母细胞瘤产生基底膜成分提供了免疫组织化学证据。
