Department of Biochemistry, West Virginia University Medical School, 26506, Morgantown, WV, USA.
Biol Trace Elem Res. 1982 Jun;4(2-3):191-7. doi: 10.1007/BF02783258.
Leukocytes incubated with Cu(II) showed a decrease in both glutathione reductase activity and reduced glutathione content. The glucose 6-phosphate dehydrogenase activity under the same conditions was not affected. Serum albumin added to mixtures prevented the loss of enzyme activity, whileD-penicillamine andL-histidine had little effect. Prior oxidation of the cell-reduced glutathione did not diminish the enzyme inhibitory action of Cu(II). The amount of regeneration of reduced glutathione in leukocytes previously treated with diamide to oxidize their reduced glutathione was a function of Cu(II) concentration in the media. No evidence was obtained that elevated serum ceruloplasmin levels in rabbits, nor incubation of leukocytes in vitro with ceruloplasmin, affect leukocyte glutathione reductase activity. It was proposed that the major mechanism by which copper affects glutathione metabolism in leukocytes is by inhibition of glutathione reductase.
白细胞与 Cu(II) 孵育后，谷胱甘肽还原酶活性和还原型谷胱甘肽含量均下降。在相同条件下，葡萄糖 6-磷酸脱氢酶的活性不受影响。添加到混合物中的血清白蛋白可防止酶活性丧失，而 D-青霉胺和 L-组氨酸的作用则很小。细胞内还原型谷胱甘肽的预先氧化并不能降低 Cu(II)对酶的抑制作用。先前用联二亚胺处理白细胞以氧化其还原型谷胱甘肽，可根据培养基中 Cu(II) 的浓度来再生还原型谷胱甘肽。没有证据表明兔血清铜蓝蛋白水平升高，或体外孵育白细胞与铜蓝蛋白，会影响白细胞谷胱甘肽还原酶的活性。据推测，铜影响白细胞谷胱甘肽代谢的主要机制是抑制谷胱甘肽还原酶。
