谷胱甘肽氧化剂对人中性粒细胞微管组装及微管依赖表面特性的影响
Effects of glutathione-oxidizing agents on microtubule assembly and microtubule-dependent surface properties of human neutrophils.
作者信息
Oliver J M, Albertini D F, Berlin R D
出版信息
J Cell Biol. 1976 Dec;71(3):921-32. doi: 10.1083/jcb.71.3.921.
Abstract
In human peripheral blood polymorphonuclear leukocytes and lymphocytes, GSH-oxidizing agents promote the movement of surface-bound concanavalin A (Con A) into caps and inhibit the assembly of microtubules (MT) that is normally induced by Con A binding. Con A capping and inhibition of MT assembly occur when GSH levels in cell suspensions are decreased by 30-70%, and return to GSH to control levels is accompanied by the appearance of cytoplasmic MT and by inhibition of the capping response with Con A. Oxidation of GSH markedly stimulates the hexose monophosphate shunt, and regeneration of GSH occurs rapidly. The data indicate that MT cannot be assembled or maintained in the face of decreased GSH levels. Thus, GSH homeostasis becomes critical during physiological events such as phagocytosis which simultaneously induce the assembly of MT and the production of agents like H2O2 that can oxidize GSH.
摘要
在人类外周血多形核白细胞和淋巴细胞中，谷胱甘肽（GSH）氧化剂可促进表面结合的伴刀豆球蛋白A（Con A）向帽状结构移动，并抑制通常由Con A结合诱导的微管（MT）组装。当细胞悬液中的GSH水平降低30%-70%时，会出现Con A帽化和MT组装抑制现象，而将GSH恢复到对照水平会伴随着细胞质MT的出现以及Con A帽化反应的抑制。GSH的氧化显著刺激磷酸己糖旁路，且GSH的再生迅速发生。数据表明，面对降低的GSH水平，MT无法组装或维持。因此，在诸如吞噬作用等生理事件中，GSH稳态变得至关重要，因为吞噬作用会同时诱导MT组装和产生如过氧化氢等可氧化GSH的物质。
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本文引用的文献
1
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Infect Immun. 1971 Jan;3(1):66-72. doi: 10.1128/iai.3.1.66-72.1971.
2
Improved method for the determination of blood glutathione.测定血液中谷胱甘肽的改进方法。
J Lab Clin Med. 1963 May;61:882-8.
3
The dissociation by colchicine of phagocytosis from increased oxygen consumption in human leukocytes.秋水仙碱使人类白细胞的吞噬作用与耗氧量增加相分离。
J Clin Invest. 1967 May;46(5):786-96. doi: 10.1172/JCI105579.
4
Suppression of metabolic accompaniments of phagocytosis by colchicine.秋水仙碱对吞噬作用代谢伴随物的抑制作用。
Arthritis Rheum. 1965 Dec;8(6):1112-22. doi: 10.1002/art.1780080610.
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Purine transport in polymorphonuclear leukocytes.多形核白细胞中的嘌呤转运
Biochim Biophys Acta. 1969 Mar 11;173(2):324-37. doi: 10.1016/0005-2736(69)90115-1.
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Role of cell surface in the regulation of oxidative metabolism of the phagocyte.细胞表面在吞噬细胞氧化代谢调节中的作用。
Acta Vitaminol Enzymol. 1973;27(1):145-50.
7
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J Infect Dis. 1973 Jan;127(1):40-8. doi: 10.1093/infdis/127.1.40.
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