Oliver J M, Albertini D F, Berlin R D
J Cell Biol. 1976 Dec;71(3):921-32. doi: 10.1083/jcb.71.3.921.
In human peripheral blood polymorphonuclear leukocytes and lymphocytes, GSH-oxidizing agents promote the movement of surface-bound concanavalin A (Con A) into caps and inhibit the assembly of microtubules (MT) that is normally induced by Con A binding. Con A capping and inhibition of MT assembly occur when GSH levels in cell suspensions are decreased by 30-70%, and return to GSH to control levels is accompanied by the appearance of cytoplasmic MT and by inhibition of the capping response with Con A. Oxidation of GSH markedly stimulates the hexose monophosphate shunt, and regeneration of GSH occurs rapidly. The data indicate that MT cannot be assembled or maintained in the face of decreased GSH levels. Thus, GSH homeostasis becomes critical during physiological events such as phagocytosis which simultaneously induce the assembly of MT and the production of agents like H2O2 that can oxidize GSH.
在人类外周血多形核白细胞和淋巴细胞中，谷胱甘肽（GSH）氧化剂可促进表面结合的伴刀豆球蛋白A（Con A）向帽状结构移动，并抑制通常由Con A结合诱导的微管（MT）组装。当细胞悬液中的GSH水平降低30%-70%时，会出现Con A帽化和MT组装抑制现象，而将GSH恢复到对照水平会伴随着细胞质MT的出现以及Con A帽化反应的抑制。GSH的氧化显著刺激磷酸己糖旁路，且GSH的再生迅速发生。数据表明，面对降低的GSH水平，MT无法组装或维持。因此，在诸如吞噬作用等生理事件中，GSH稳态变得至关重要，因为吞噬作用会同时诱导MT组装和产生如过氧化氢等可氧化GSH的物质。
