Department of Chemistry, Miami University, 45056, Oxford, Ohio.
J Chem Ecol. 1989 Apr;15(4):1335-47. doi: 10.1007/BF01014834.
A method has been developed for detecting tannin-binding proteins in the saliva of herbivores. The method is simple and requires only small quantities of crude saliva. The saliva of deer, a browsing ruminant, has been compared to that of domestic sheep and cow, which are grazing ruminants. The browser, which normally ingests dietary tannin, produces tannin-binding proteins, while the grazers do not produce such proteins. The tannin-binding protein from deer saliva is a small glycoprotein containing large amounts of proline, glycine, and glutamate/glutamine. The protein is not closely related to the proline-rich salivary proteins found in rats and other nonruminant mammals.
已经开发出一种方法来检测草食动物唾液中的单宁结合蛋白。该方法简单，只需要少量粗唾液。已将鹿（一种食草反刍动物）的唾液与绵羊和奶牛（放牧反刍动物）的唾液进行了比较。通常摄入饮食单宁的食草动物会产生单宁结合蛋白，而放牧动物则不会产生这种蛋白。鹿唾液中的单宁结合蛋白是一种含有大量脯氨酸、甘氨酸和谷氨酸/谷氨酰胺的小糖蛋白。该蛋白与在大鼠和其他非反刍哺乳动物中发现的富含脯氨酸的唾液蛋白没有密切关系。
