Artiss J D, Epstein E, Kiechle F L, Zak B
Clin Lab Med. 1986 Sep;6(3):427-40.
Potential problems are described that one could encounter in carrying out an electrophoretic procedure including its ancillary phases of visualization (staining) and quantification (densitometry). Endpoint-like measurements for separated isoenzymes may provide artifactual kinetic values as well, because stain measurement is fixed at a single time whereas reagent blanking in the electrophoretic medium is substituted for the conventional serum initial absorbance readings of test-tube determinations. Truncation of separated electrophoretic zones or opacity of an electrophoretic anti-convection medium such as uncleared cellulose acetate may also interfere with absolute quantification procedures.
文中描述了在进行电泳程序（包括其可视化（染色）和定量（光密度测定）的辅助阶段）时可能遇到的潜在问题。分离的同工酶的终点样测量也可能提供人为的动力学值，因为染色测量是在单一时间固定的，而电泳介质中的试剂空白替代了试管测定中常规的血清初始吸光度读数。分离的电泳区带截断或电泳抗对流介质（如未澄清的醋酸纤维素）不透明也可能干扰绝对定量程序。
