Zak B, Baginski E S, Epstein E
Ann Clin Lab Sci. 1978 Sep-Oct;8(5):385-95.
The process of electrophoresis, a separation phenomenon, is mistakenly understood to include the sequential processes ancillary to analyte resolution, that is, staining and quantification, where the latter could be elution followed by photometry or integrating-calculating-densitometry. The theories involved in electrophoresis itself are well worked out and equally well understood but the problems which are associated with separation, chemical reaction to generate a chromogen and quantification, perhaps partly forgotten and perhaps partly ignored, are taken up and described here. They include albumin trail, resolution, unequivalent staining, prestaining and the densitometry problems associated with band widths, opacity effects and polychromaticities.
电泳作为一种分离现象，其过程被错误地理解为包括与分析物分离相关的一系列辅助过程，即染色和定量分析，其中后者可能是洗脱后进行光度测定或积分计算密度测定。电泳本身所涉及的理论已经得到了充分的研究且人们也有很好的理解，但这里将探讨并描述与分离、产生发色团的化学反应以及定量分析相关的问题，这些问题可能部分被遗忘，部分被忽视。它们包括白蛋白拖尾、分离度、不等量染色、预染色以及与带宽、不透明度效应和多色性相关的密度测定问题。
