Nagamori H, Ohno Y, Uchima E, Kajiwara M, Nakazato M, Une Y, Takeda K
Forensic Sci Int. 1986 Jun;31(2):119-28. doi: 10.1016/0379-0738(86)90195-7.
In order to determine sex using a single specimen, buccal mucosa and hair roots obtained from male and female individuals were used. The specimens were first stained with quinacrine for the detection of the Y-chromatin, and subsequently were stained by the fluorescent Feulgen reaction using acriflavine for the detection of the X-chromatin. In the male specimens, the frequency of fluorescent spots of quinacrine-positive bodies was high, whereas that of acriflavine-positive spots was low. On the other hand, in the female specimens, the frequency of quinacrine-positive spots was very low, while that of the acriflavine-positive spots was high. These specimens were air-dried and were allowed to stand at room temperature for periodical observations. The result was that sex difference was distinguishable for 4 months by the combined treatment method.
为了使用单个样本确定性别，我们使用了从男性和女性个体获取的颊黏膜和发根。样本首先用喹吖因染色以检测Y染色质，随后用吖啶黄通过荧光福尔根反应染色以检测X染色质。在男性样本中，喹吖因阳性体的荧光斑点频率较高，而吖啶黄阳性斑点的频率较低。另一方面，在女性样本中，喹吖因阳性斑点的频率非常低，而吖啶黄阳性斑点的频率较高。这些样本经空气干燥后，在室温下放置以便进行定期观察。结果表明，通过联合处理方法在4个月内可区分性别差异。
