Pappalardo G, Pelotti S
Istituto di medicina legale e delle assicurazioni Università, Bologna.
Quad Sclavo Diagn. 1988 Jan-Dec;24(1-4):29-36.
Japanese authors have described a procedure for the sex determination of human hair roots without epithelial sheath, paraffin embedded and fluorescent stained for cortical cell nuclei Y or X sex chromatins. This method however is not sufficiently practical for forensic science purposes: it is difficult and not guaranteed to obtain suitable longitudinal hair sections with possible loss of evidence particularly serious when the questioned hair is only one. The authors, researching Y-bodies with Quinacrine, present an alternative technique of preparation of specimens without embedding which is simple and quick, reproducible and satisfying in results and that avoids loss of material.
日本学者描述了一种用于人类无根毛囊性别鉴定的方法，该毛囊经过石蜡包埋，并对皮质细胞核的Y或X性染色质进行荧光染色。然而，这种方法在法医学应用中实用性不足：获取合适的毛发纵切片难度较大且无法保证，特别是在可疑毛发仅有一根时，证据丢失的可能性尤为严重。作者们在用喹吖因研究Y小体时，提出了一种无需包埋的标本制备替代技术，该技术简单快捷、可重复且结果令人满意，还能避免材料丢失。
