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磷酸烯醇丙酮酸羧化酶在结瘤桤木中的免疫细胞化学研究。

Immunocytochemical study of phosphoenolpyruvate carboxylase in nodulated Alnus glutinosa.

机构信息

Laboratoire de Biologie Végétale, Université de Nancy I, ERA au CNRS no 799, C/O no 140, F-54037, Nancy Cedex, France.

出版信息

Planta. 1981 Oct;153(1):14-7. doi: 10.1007/BF00385312.

DOI:10.1007/BF00385312
PMID:24276701
Abstract

The activities of phosphoenolpyruvate carboxylase (PEP carboxylase, EC 4.1.1.3.1) have been investigated in various organs of young nodulated Alnus glutinosa. The root nodules exhibited the highest specific enzyme activity when compared with the one in roots and leaves. Furthermore, in the root nodules the PEP carboxylase was predominantly localized in the cytosol of the large cortical cells containing the endophyte vesicles.

摘要

已研究了磷酸烯醇丙酮酸羧化酶(PEP 羧化酶,EC 4.1.1.3.1)在幼龄结瘤桤木的各种器官中的活性。与根和叶中的酶活性相比,根瘤表现出最高的比酶活。此外,在根瘤中,PEP 羧化酶主要定位于含有内生菌囊泡的大皮层细胞的细胞质中。

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引用本文的文献

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In-situ immunofluorescent localization of phosphoenolpyruvate and ribulose 1,5-bisphosphate carboxylases in leaves of C3, C 4, and C 3-C 4 intermediatePanicum species.在 C3、C4 和 C3-C4 中间型 Panicum 物种叶片中磷酸烯醇丙酮酸和核酮糖 1,5-二磷酸羧化酶的原位免疫荧光定位。
Planta. 1984 May;161(3):266-71. doi: 10.1007/BF00982924.
2
Phosphoenolpyruvate carboxylase in soybean root nodules: An immunochemical study.大豆根瘤中的磷酸烯醇丙酮酸羧化酶:免疫化学研究。
Planta. 1986 Oct;169(2):198-201. doi: 10.1007/BF00392314.

本文引用的文献

1
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Planta. 1981 Mar;151(3):226-31. doi: 10.1007/BF00395173.
2
Carbon Dioxide Fixation by Lupin Root Nodules: I. Characterization, Association with Phosphoenolpyruvate Carboxylase, and Correlation with Nitrogen Fixation during Nodule Development.羽扇豆根瘤的二氧化碳固定作用:I. 特性、与磷酸烯醇式丙酮酸羧化酶的关联以及在根瘤发育过程中与固氮作用的相关性
Plant Physiol. 1977 Jul;60(1):47-50. doi: 10.1104/pp.60.1.47.
3
CO(2) Metabolism in Corn Roots. II. Intracellular Distribution of Enzymes.
玉米根中的 CO2 代谢。Ⅱ. 酶的细胞内分布。
Plant Physiol. 1967 May;42(5):719-24. doi: 10.1104/pp.42.5.719.
4
Localization of antigen in tissue cells; improvements in a method for the detection of antigen by means of fluorescent antibody.组织细胞中抗原的定位;通过荧光抗体检测抗原的方法的改进。
J Exp Med. 1950 Jan 1;91(1):1-13. doi: 10.1084/jem.91.1.1.
5
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
6
A simple method for removing the resin from epoxy-embedded tissue.一种从环氧树脂包埋组织中去除树脂的简单方法。
J Biophys Biochem Cytol. 1961 Apr;9(4):909-10. doi: 10.1083/jcb.9.4.909.
7
Phosphoenolpyruvate carboxylase from soybean nodule cytosol. Evidence for isoenzymes and kinetics of the most active component.来自大豆根瘤细胞质的磷酸烯醇丙酮酸羧化酶。同工酶的证据及最具活性组分的动力学研究。
Biochim Biophys Acta. 1979 Apr 12;567(2):445-52. doi: 10.1016/0005-2744(79)90130-x.