Calgene, Inc., 1920 Fifth Street, 95616, Davis, CA, USA.
Plant Mol Biol. 1987 Mar;9(2):127-34. doi: 10.1007/BF00015645.
A 715 base pair cDNA clone coding for an acyl carrier protein (ACP) in spinach leaves has been isolated and characterized. The amino acid sequence indicated by the cDNA sequence closely matches the amino acid sequence of the ACP-I isoform. The presence of polyadenylation and DNA sequence coding for a precursor protein with a putative transit peptide, and the absence of hybridization between the cloned DNA and isolated spinach plastid DNA collectively show that the ACP-I gene is nuclear-encoded. The ACP-I cloned DNA did not cross-hybridize with mRNA from spinach tissues in which ACP-II has been found. Cross-hybridization with mRNA from tissues of Brassica campestris was either weak or undetectable. The cloning of an ACP-I gene represents an initial step in the molecular dissection of fatty acid synthetase in plants.
已分离并鉴定出编码菠菜叶酰基辅酶 A 蛋白（ACP）的 715 个碱基对 cDNA 克隆。cDNA 序列所指示的氨基酸序列与 ACP-I 同工型的氨基酸序列非常吻合。多聚腺苷酸化的存在以及编码具有假定转运肽的前体蛋白的 DNA 序列，以及克隆 DNA 与分离的菠菜质体 DNA 之间不存在杂交，这些都表明 ACP-I 基因是核编码的。ACP-I 克隆 DNA 与已发现 ACP-II 的菠菜组织的 mRNA 不发生杂交。与 Brassica campestris 组织的 mRNA 发生交叉杂交的程度很弱或无法检测到。ACP-I 基因的克隆代表了植物脂肪酸合成酶分子剖析的初始步骤。
