Department of Clinical Laboratory Science, College of Health and Therapy, Daegu Haany University, Gyeongsan 712-715, Korea.
Toxicol Res. 2011 Jun;27(2):95-102. doi: 10.5487/TR.2011.27.2.095.
The effect of DHU001, a mixed herbal formula consisted of 7 types aqueous extracts for various respiratory disorders were evaluated on the formalin-induced paw chronic inflammation in mice after oral administration. Mice were subaponeurotically injected in the left hind paw with 0.02 ml of 3.75% formalin, then subjected to 500, 250 and 125 mg/kg of DHU001 oral administration, once a day for 10 days during which then the hind-paw thickness and volume were measured daily. The paw wet-weight, histological profiles, histomorphometrical analyses and paw tumor necrosis factor (TNF)-α contents were conducted at termination. After two formalin treatments, a marked increase in the paw thickness and volume was detected in the formalin-injected control as compared with that in the intact control, plus at the time of sacrifice the paw wet-weights, paw TNF-α contents were also dramatically increased with severe chronic inflammation signs at histopathological observations. However, these formalin-induced chronic inflammatory changes were dramatically decreased by treatment of dexamethasone and all three different dosages of DHU001. DHU001 has favorable effects on formalin-induced chronic inflammation mediated by TNF-α suppression, and DHU001 may represent an alternative approach for the treatment of chronic inflammatory diseases.
一种由 7 种不同植物水提物组成的复方草药 DHU001 对甲醛诱导的慢性炎症的影响。将 0.02ml3.75%甲醛亚急性注入小鼠左后足底皮下，造模成功后，DHU001 高、中、低剂量组（500、250、125mg/kg）灌胃给药，1 次/天，连续 10 天，每天测量后足肿胀度和容积。末次给药后检测小鼠足爪湿重、组织学形态、组织形态计量学和肿瘤坏死因子（TNF）-α含量。两次甲醛刺激后，与空白对照组相比，甲醛诱导的对照组小鼠后足肿胀度和容积显著增加，处死时，湿重和 TNF-α含量也显著增加，组织病理学观察到严重的慢性炎症迹象。然而，地塞米松和 DHU001 的三种不同剂量均可显著降低甲醛诱导的慢性炎症变化。DHU001 对 TNF-α 抑制介导的甲醛诱导的慢性炎症具有良好的作用，DHU001 可能为治疗慢性炎症性疾病提供一种替代方法。
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