Department of Molecular Biotechnology, Ghent University, Coupure links 653, B-9000, Ghent, Belgium.
Mol Plant Pathol. 2014 May;15(4):352-63. doi: 10.1111/mpp.12098. Epub 2013 Nov 27.
The rice root nematode Hirschmanniella oryzae is the most abundant plant-parasitic nematode in flooded rice fields and is distributed world-wide. Although it is economically less important than sedentary nematodes, it can cause severe yield reductions and economic losses in specific environmental conditions. No transcriptome data for this genus were available until now. We have performed 454 sequencing on a mixed life stages population to gain an insight into nematode-plant interactions and nematode survival strategies. The results of two assembly strategies were combined to reduce the redundancy of the data, generating a final dataset of 21 360 contigs. The data were screened for putative plant cell wall-modifying proteins, which facilitate nematode migration through host roots. A β-mannanase, previously not reported in nematodes, was detected in the dataset. The data were screened for putative effector proteins that may alter the host defence mechanism. Two enzymes, chorismate mutase and isochorismatase, thought to be involved in the salicyclic acid pathway, were identified. Experimental treatments of H. oryzae with artificial seawater showed that late embryogenesis abundant (LEA) proteins and SXP/RAL-2 are induced, suggesting that these proteins are involved in the process of anhydrobiosis. The newly generated data can highlight potential differences between sedentary and migratory nematodes, and will be useful in the further study of host-nematode interactions and the developmental biology of this nematode.
水稻根结线虫 Hirschmanniella oryzae 是稻田中最丰富的植物寄生线虫，分布于世界各地。尽管它在经济上不如固着型线虫重要，但在特定的环境条件下，它会导致严重的减产和经济损失。到目前为止，还没有该属的转录组数据。我们对混合生活阶段的种群进行了 454 测序，以深入了解线虫-植物相互作用和线虫的生存策略。两种组装策略的结果相结合，减少了数据的冗余，生成了最终数据集，包含 21360 个连续序列。对数据进行筛选，寻找可能有助于线虫通过宿主根迁移的植物细胞壁修饰蛋白。在数据集检测到一种 β-甘露聚糖酶，这种酶以前未在线虫中报道过。对可能改变宿主防御机制的效应蛋白进行了筛选。鉴定出两种酶，即色氨酸合酶和异色氨酸酶，它们被认为参与水杨酸途径。用人工海水处理水稻根结线虫的实验表明，晚期胚胎丰富蛋白（LEA）和 SXP/RAL-2 被诱导，表明这些蛋白参与了脱水休眠过程。新生成的数据可以突出固着型和迁移型线虫之间的潜在差异，并有助于进一步研究宿主-线虫相互作用和这种线虫的发育生物学。
