Center of Basic Research II, Biomedical Research Foundation of the Academy of Athens, 4 Soranou Ephessiou, 11527 Athens, Greece.
Appl Microbiol Biotechnol. 2013 Mar;97(5):2109-18. doi: 10.1007/s00253-012-4357-9. Epub 2012 Aug 26.
This study examined the effects of three lactic acid bacteria (LAB) strains on the nematode Caenorhabditis elegans. Lactobacillus salivarius, Lactobacillus reuteri, and Pediococcus acidilactici were found to inhibit the development and growth of the worm. Compared to Escherichia coli used as the control, L. reuteri and P. acidilactici reduced the lifespan of wild-type and short-lived daf-16 worms. On the contrary, L. salivarius extended the lifespan of daf-16 worms when used live, but reduced it as UV-killed bacteria. The three LAB induced the expression of genes involved in pathogen response and inhibited the growth of tumor-like germ cells, without affecting DAF16 localization or increasing corpse cells. Our results suggest the possible use of C. elegans as a model for studying the antitumor attributes of LAB. The negative effects of these LAB strains on the nematode also indicate their potential use against parasitic nematodes.
本研究探讨了三种乳酸菌（LAB）菌株对秀丽隐杆线虫的影响。发现唾液乳杆菌、雷特氏乳杆菌和嗜酸乳球菌抑制了蠕虫的发育和生长。与用作对照的大肠杆菌相比，L. reuteri 和 P. acidilactici 缩短了野生型和短命 daf-16 蠕虫的寿命。相反，当使用活菌时，L. salivarius 延长了 daf-16 蠕虫的寿命，但作为 UV 杀死的细菌时，其寿命缩短。三种 LAB 诱导了参与病原体反应的基因的表达，并抑制了肿瘤样生殖细胞的生长，而不影响 DAF16 的定位或增加尸体细胞。我们的研究结果表明，秀丽隐杆线虫可能被用作研究 LAB 抗肿瘤特性的模型。这些 LAB 菌株对线虫的负面影响也表明它们可能被用于对抗寄生线虫。
