泌乳期牛乳腺磷酸丝氨酰tRNA的特性分析。
The characterization of phosphoseryl tRNA from lactating bovine mammary gland.
作者信息
Sharp S J, Stewart T S
出版信息
Nucleic Acids Res. 1977 Jul;4(7):2123-36. doi: 10.1093/nar/4.7.2123.
Abstract
BD-cellulose and RPC-5 chromatography of tRNA isolated from lactating bovine mammary gland showed the presence of four seryl-tRNA isoacceptors. The species, tRNA IV Ser, with the strongest affinity for BD-cellulose (required ethanol in the elution buffer) could be phosphorylated in the presence of serine, [gamma-32 P]-ATP, seryl-tRNA synthetase and phosphotransferase activity from the same tissue. O-Phosphoserine was identified as the 32P-labelled product after mild alkaline hydrolysis of this aminoacylated tRNA. Pancreatic ribonuclease treatment of the aminoacylated tRNA yielded a labelled product which was identified as phosphoseryladenosine. These results indicated there is a specific phosphoseryl tRNA species in lactating bovine mammary gland. It appears that the formation of phosphoseryl-tRNA proceeds by enzymic phosphorylation of seryl-tRNA.
摘要
从泌乳牛乳腺中分离出的tRNA经BD-纤维素和RPC-5色谱分析表明存在四种丝氨酰-tRNA同工受体。对BD-纤维素亲和力最强的tRNA IV Ser（洗脱缓冲液中需要乙醇）在存在丝氨酸、[γ-32P]-ATP、丝氨酰-tRNA合成酶和来自同一组织的磷酸转移酶活性的情况下可以被磷酸化。该氨酰化tRNA经温和碱性水解后，O-磷酸丝氨酸被鉴定为32P标记产物。用胰核糖核酸酶处理该氨酰化tRNA产生一种标记产物，该产物被鉴定为磷酸丝氨酰腺苷。这些结果表明泌乳牛乳腺中存在一种特定的磷酸丝氨酰tRNA。磷酸丝氨酰-tRNA的形成似乎是通过丝氨酰-tRNA的酶促磷酸化进行的。
相似文献
1
The characterization of phosphoseryl tRNA from lactating bovine mammary gland.泌乳期牛乳腺磷酸丝氨酰tRNA的特性分析。
Nucleic Acids Res. 1977 Jul;4(7):2123-36. doi: 10.1093/nar/4.7.2123.
2
Possible incorporation of phosphoserine into globin readthrough protein via bovine opal suppressor phosphoseryl-tRNA.通过牛的乳白抑制子磷酸丝氨酰 - tRNA将磷酸丝氨酸掺入珠蛋白通读蛋白的可能性。
FEBS Lett. 1986 Oct 20;207(1):162-6. doi: 10.1016/0014-5793(86)80032-1.
3
pGp as the main product of bovine tRNA kinase.P糖蛋白作为牛源tRNA激酶的主要产物。
Mol Biol Rep. 2002 Sep;29(3):293-300. doi: 10.1023/a:1020423705963.
4
Identification and characterization of phosphoseryl-tRNA[Ser]Sec kinase.磷酸丝氨酰 - tRNA[Ser]Sec激酶的鉴定与特性分析
Proc Natl Acad Sci U S A. 2004 Aug 31;101(35):12848-53. doi: 10.1073/pnas.0402636101. Epub 2004 Aug 18.
5
A study of the interaction between tRNASer and seryl-tRNA synthetase from bovine liver.一项关于来自牛肝脏的tRNASer与丝氨酰-tRNA合成酶之间相互作用的研究。
Nucleic Acids Symp Ser. 1985(16):217-20.
6
[Purification and properties of leucyl-tRNA synthetase from the cow mammary gland].[奶牛乳腺亮氨酰 - tRNA合成酶的纯化及性质]
Mol Biol (Mosk). 1979 May-Jun;13(3):550-8.
7
A specific hepatic transfer RNA for phosphoserine.一种特定的磷酸丝氨酸肝转运RNA。
Proc Natl Acad Sci U S A. 1970 Oct;67(2):688-95. doi: 10.1073/pnas.67.2.688.
8
[Isolation and characterization of individual tRNA Leu 1,2,and 4 from the bovine udder].[从牛乳房中分离并鉴定单个亮氨酰tRNA 1、2和4]
Biokhimiia. 1983 Dec;48(12):1984-7.
9
Biosynthesis of selenocysteine on its tRNA in eukaryotes.真核生物中硒代半胱氨酸在其转运核糖核酸上的生物合成。
PLoS Biol. 2007 Jan;5(1):e4. doi: 10.1371/journal.pbio.0050004.
10
Evidence for the absence of the terminal adenine nucleotide at the amino acid-acceptor end of transfer ribonucleic acid in non-lactating bovine mammary gland and its inhibitory effect on the aminoacylation of rat liver transfer ribonucleic acid.非泌乳期奶牛乳腺中转运核糖核酸氨基酸受体末端不存在末端腺嘌呤核苷酸的证据及其对大鼠肝脏转运核糖核酸氨酰化的抑制作用。
Biochem J. 1970 Feb;116(3):405-14. doi: 10.1042/bj1160405.
引用本文的文献
1
Selenocysteine tRNA, the Central Component of Selenoprotein Biosynthesis: Isolation, Identification, Modification, and Sequencing.硒代半胱氨酸转运RNA，硒蛋白生物合成的核心组成部分：分离、鉴定、修饰及测序
Methods Mol Biol. 2018;1661:43-60. doi: 10.1007/978-1-4939-7258-6_4.
2
Distinct genetic code expansion strategies for selenocysteine and pyrrolysine are reflected in different aminoacyl-tRNA formation systems.用于硒代半胱氨酸和吡咯赖氨酸的独特遗传密码扩展策略反映在不同的氨酰-tRNA形成系统中。
FEBS Lett. 2010 Jan 21;584(2):342-9. doi: 10.1016/j.febslet.2009.11.005.
3
Amino acid modifications on tRNA.转运核糖核酸（tRNA）上的氨基酸修饰
Acta Biochim Biophys Sin (Shanghai). 2008 Jul;40(7):539-53. doi: 10.1111/j.1745-7270.2008.00435.x.
4
Divergence of selenocysteine tRNA recognition by archaeal and eukaryotic O-phosphoseryl-tRNASec kinase.古细菌和真核生物O-磷酸丝氨酰-tRNASec激酶对硒代半胱氨酸tRNA识别的差异
Nucleic Acids Res. 2008 Apr;36(6):1871-80. doi: 10.1093/nar/gkn036. Epub 2008 Feb 11.
5
From one amino acid to another: tRNA-dependent amino acid biosynthesis.从一种氨基酸到另一种氨基酸：依赖于转运RNA的氨基酸生物合成
Nucleic Acids Res. 2008 Apr;36(6):1813-25. doi: 10.1093/nar/gkn015. Epub 2008 Feb 5.
6
Characterization and evolutionary history of an archaeal kinase involved in selenocysteinyl-tRNA formation.参与硒代半胱氨酰-tRNA形成的古菌激酶的特性及进化史
Nucleic Acids Res. 2008 Mar;36(4):1247-59. doi: 10.1093/nar/gkm1134. Epub 2008 Jan 3.
7
Structural insights into RNA-dependent eukaryal and archaeal selenocysteine formation.对依赖RNA的真核生物和古细菌硒代半胱氨酸形成的结构见解。
Nucleic Acids Res. 2008 Mar;36(4):1187-99. doi: 10.1093/nar/gkm1122. Epub 2007 Dec 23.
8
RNA-dependent conversion of phosphoserine forms selenocysteine in eukaryotes and archaea.在真核生物和古细菌中，磷酸丝氨酸的RNA依赖性转化形成硒代半胱氨酸。
Proc Natl Acad Sci U S A. 2006 Dec 12;103(50):18923-7. doi: 10.1073/pnas.0609703104. Epub 2006 Dec 1.
9
Phosphate incorporation into secretory protein of Chironomus salivary glands occurs during translation.磷酸根掺入到摇蚊唾液腺分泌蛋白的过程发生在翻译过程中。
EMBO J. 1984 Dec 1;3(12):2851-5. doi: 10.1002/j.1460-2075.1984.tb02219.x.
10
On the road to selenocysteine.通往硒代半胱氨酸之路。
Proc Natl Acad Sci U S A. 2004 Sep 14;101(37):13395-6. doi: 10.1073/pnas.0405357101. Epub 2004 Sep 7.
本文引用的文献
1
TRANSFER RIBONUCLEIC ACIDS.转移核糖核酸
Nature. 1964 Jun 6;202:984-6. doi: 10.1038/202984a0.
2
The formation of hydroxypyruvyl-tRNA.羟基丙酮酸 - 转运RNA的形成。
FEBS Lett. 1969 Aug;4(3):222-226. doi: 10.1016/0014-5793(69)80240-1.
3
Post-translational phosphorylation of phosvitin.卵黄高磷蛋白的翻译后磷酸化
FEBS Lett. 1973 Mar 1;30(2):167-169. doi: 10.1016/0014-5793(73)80643-x.
4
The separation of soluble ribonucleic acids on benzoylated diethylaminoethylcellulose.在苯甲酰化二乙氨基乙基纤维素上分离可溶性核糖核酸。
Biochemistry. 1967 Oct;6(10):3043-56. doi: 10.1021/bi00862a011.
5
Casein biosynthesis: evidence for phosphorylation of precursor proteins.酪蛋白生物合成：前体蛋白磷酸化的证据。
Biochim Biophys Acta. 1966 Oct 31;127(2):366-72. doi: 10.1016/0304-4165(66)90391-6.
6
A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity.一种制备高比活度32-P标记三磷酸腺苷的简单方法。
Biochem J. 1964 Jan;90(1):147-9. doi: 10.1042/bj0900147.
7
8
Quantitative variation in serine transfer ribonucleic acid during estrogen-induced phosphoprotein synthesis in rooster liver.雌激素诱导公鸡肝脏磷蛋白合成过程中丝氨酸转移核糖核酸的定量变化。
Biochemistry. 1969 Dec;8(12):4926-35. doi: 10.1021/bi00840a041.
9
Transfer RNA as a cofactor coupling amino acid synthesis with that of protein.转运RNA作为一种辅助因子，将氨基酸合成与蛋白质合成联系起来。
Proc Natl Acad Sci U S A. 1968 Sep;61(1):229-36. doi: 10.1073/pnas.61.1.229.
10
Columns for rapid chromatographic separation of small amounts of tracer-labeled transfer ribonucleic acids.用于快速色谱分离少量示踪剂标记的转移核糖核酸的柱。
Anal Biochem. 1971 Dec;44(2):486-95. doi: 10.1016/0003-2697(71)90236-3.
Zotero 插件