Dalton S, Younghusband H B, Wells J R
Nucleic Acids Res. 1986 Aug 26;14(16):6507-23. doi: 10.1093/nar/14.16.6507.
The association between histone genes and the nuclear matrix (NM) during periods of high (S-phase) and low (non-S-phase) transcriptional activity has been investigated with synchronized cells from a chicken erythroid cell line (abbreviated ts34). By DNase I and restriction enzyme analysis, these studies reveal that both core and linker histone genes (represented by H2A and H1 genes respectively) are attached to the NM independent of their transcriptional activity during the cell-cycle. The tissue-specific histone gene H5, expressed constitutively, is nuclear matrix (NM)-associated in ts34 cells but is found in the supernatant (S/N) fractions of a non-erythroid T-cell line. Furthermore, we show that DNA sequences necessary for NM-attachment of the H5 gene lie within a 780 base pair region spanning part of the coding and 5' non-translated region. Of the three non-histone genes investigated, beta-actin sequences are expressed and are NM-attached, feather keratin genes are not expressed and predominate in the S/N, and beta-globin genes although not expressed in the ts34 cell line used were found in the NM fraction. In this case the association may be fortuitous or may reflect an early event prior to transcription of globin genes in differentiating erythroid cells. These results generally support the notion that actively transcribed genes are NM-attached, but that attachment per se is not synonymous with transcription.
利用来自鸡红细胞系（简称ts34）的同步化细胞，研究了在高转录活性（S期）和低转录活性（非S期）期间组蛋白基因与核基质（NM）之间的关联。通过DNase I和限制性酶分析，这些研究表明，核心组蛋白基因和连接组蛋白基因（分别由H2A和H1基因代表）在细胞周期中均独立于其转录活性而附着于核基质。组成性表达的组织特异性组蛋白基因H5在ts34细胞中与核基质（NM）相关，但在非红细胞T细胞系的上清液（S/N）组分中发现。此外，我们表明H5基因与核基质附着所需的DNA序列位于跨越部分编码区和5'非翻译区的780个碱基对区域内。在所研究的三个非组蛋白基因中，β-肌动蛋白序列表达且与核基质附着，羽毛角蛋白基因不表达且在上清液（S/N）中占主导，β-珠蛋白基因虽然在所使用的ts34细胞系中不表达，但在核基质组分中发现。在这种情况下，这种关联可能是偶然的，或者可能反映了分化红细胞中珠蛋白基因转录之前的早期事件。这些结果总体上支持了这样一种观点，即活跃转录的基因与核基质附着，但附着本身并不等同于转录。
