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鸡红细胞的核基质DNA含有β-珠蛋白基因序列。

Nuclear matrix DNA from chicken erythrocytes contains beta-globin gene sequences.

作者信息

Hentzen P C, Rho J H, Bekhor I

出版信息

Proc Natl Acad Sci U S A. 1984 Jan;81(2):304-7. doi: 10.1073/pnas.81.2.304.

DOI:10.1073/pnas.81.2.304
PMID:6582488
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC344664/
Abstract

Nuclear matrices containing residual DNA were isolated from chicken erythrocytes after extraction of purified nuclei with buffered 2 M NaCl. After further purification of this residual DNA, it was found to contain high concentrations of beta-globin gene sequences as assayed by dot hybridization with 32P-labeled nick-translated pHB1001. Electron microscopy of a random sample of this residual DNA fraction shows the DNA to be intimately associated with protein at various intervals. A hypothesis for enrichment of active genes in residual DNA from purified chromatin or in nuclear matrix DNA is also discussed.

摘要

在用含缓冲液的2M氯化钠提取纯化细胞核后，从鸡红细胞中分离出含有残留DNA的核基质。对这种残留DNA进一步纯化后，通过与32P标记的缺口平移pHB1001进行点杂交分析发现，其含有高浓度的β-珠蛋白基因序列。对该残留DNA部分的随机样本进行电子显微镜观察，结果显示DNA在不同间隔处与蛋白质紧密相连。文中还讨论了从纯化染色质的残留DNA或核基质DNA中富集活性基因的假说。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/500f/344664/98c4646ac0af/pnas00603-0025-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/500f/344664/235e8f71bb10/pnas00603-0024-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/500f/344664/a0935f3da8f5/pnas00603-0024-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/500f/344664/9536b47e3180/pnas00603-0024-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/500f/344664/98c4646ac0af/pnas00603-0025-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/500f/344664/235e8f71bb10/pnas00603-0024-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/500f/344664/a0935f3da8f5/pnas00603-0024-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/500f/344664/9536b47e3180/pnas00603-0024-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/500f/344664/98c4646ac0af/pnas00603-0025-a.jpg

相似文献

1
Nuclear matrix DNA from chicken erythrocytes contains beta-globin gene sequences.鸡红细胞的核基质DNA含有β-珠蛋白基因序列。
Proc Natl Acad Sci U S A. 1984 Jan;81(2):304-7. doi: 10.1073/pnas.81.2.304.
2
[Localization of regions of DNA attachment to the nuclear skeleton within chicken alpha-globin genes in functionally active and functionally inactive nuclei].[在功能活跃和功能不活跃细胞核内鸡α-珠蛋白基因中与核骨架相连的DNA区域的定位]
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3
Distribution of tightly bound proteins in the chicken ovalbumin gene region.紧密结合蛋白在鸡卵清蛋白基因区域的分布
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4
Comparison of the folding of beta-globin and ovalbumin gene containing chromatin isolated from chicken oviduct and erythrocytes.从鸡输卵管和红细胞中分离出的含β-珠蛋白和卵清蛋白基因的染色质折叠情况的比较。
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The beta-globin domain in immature chicken erythrocytes: enhanced solubility is coincident with histone hyperacetylation.未成熟鸡红细胞中的β-珠蛋白结构域：溶解性增强与组蛋白高度乙酰化同时发生。
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Release of a globin gene enriched chromatin fraction from chicken erythrocyte nuclei following DNase II digestion.经脱氧核糖核酸酶II消化后，从鸡红细胞核中释放出富含珠蛋白基因的染色质组分。
Nucleic Acids Res. 1981 Dec 11;9(23):6471-85. doi: 10.1093/nar/9.23.6471.
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Reactivation of chicken erythrocyte nuclei in heterokaryons results in expression of adult chicken globin genes.异核体中鸡红细胞核的重新激活导致成年鸡珠蛋白基因的表达。
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Characterization of DNA pattern in the site of permanent attachment to the nuclear matrix located in the vicinity of replication origin.位于复制起点附近的与核基质永久附着位点的DNA模式特征分析。
Biochem Biophys Res Commun. 1990 Apr 16;168(1):9-15. doi: 10.1016/0006-291x(90)91667-h.

引用本文的文献

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Association of transcriptionally active vitellogenin II gene with the nuclear matrix of chicken liver.转录活性卵黄蛋白原II基因与鸡肝核基质的关联
EMBO J. 1984 Sep;3(9):2005-8. doi: 10.1002/j.1460-2075.1984.tb02082.x.
2
Localization of specific rDNA spacer sequences to the mouse L-cell nucleolar matrix.特定核糖体DNA间隔序列在小鼠L细胞核仁基质中的定位。
Mol Cell Biol. 1985 Jun;5(6):1287-94. doi: 10.1128/mcb.5.6.1287-1294.1985.
3
N-Butyrate incubation of immature chicken erythrocytes preferentially enhances the solubility of beta A chromatin.

本文引用的文献

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A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.用于比色法测定脱氧核糖核酸的二苯胺反应的条件及机制研究。
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Erythroid specific nuclear antigen and globin gene binding protein.红系特异性核抗原和珠蛋白基因结合蛋白。
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The specific binding of estrogens and androgens to the nuclear matrix of sex hormone responsive tissues.
用正丁酸盐培养未成熟鸡红细胞可优先提高βA染色质的溶解度。
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A constitutively transcribed actin gene is associated with the nuclear matrix in a Drosophila cell line.一个组成型转录的肌动蛋白基因与果蝇细胞系中的核基质相关联。
In Vitro Cell Dev Biol. 1985 Nov;21(11):641-8. doi: 10.1007/BF02623297.
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Highly recurring sequence elements identified in eukaryotic DNAs by computer analysis are often homologous to regulatory sequences or protein binding sites.通过计算机分析在真核生物DNA中鉴定出的高度重复序列元件通常与调控序列或蛋白质结合位点同源。
Nucleic Acids Res. 1987 Feb 25;15(4):1835-51. doi: 10.1093/nar/15.4.1835.
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Alterations in nuclear matrix structure after adenovirus infection.腺病毒感染后核基质结构的改变。
J Virol. 1987 Apr;61(4):1007-18. doi: 10.1128/JVI.61.4.1007-1018.1987.
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Transcription of the histone H5 gene is not S-phase regulated.组蛋白H5基因的转录不受S期调控。
Mol Cell Biol. 1986 Feb;6(2):601-6. doi: 10.1128/mcb.6.2.601-606.1986.
8
The nonchromatin substructures of the nucleus: the ribonucleoprotein (RNP)-containing and RNP-depleted matrices analyzed by sequential fractionation and resinless section electron microscopy.细胞核的非染色质亚结构：通过连续分级分离和无树脂切片电子显微镜分析的含核糖核蛋白（RNP）和不含RNP的基质。
J Cell Biol. 1986 May;102(5):1654-65. doi: 10.1083/jcb.102.5.1654.
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Ribosomal DNA sequences attached to the nuclear matrix.附着于核基质的核糖体DNA序列。
Biochem Genet. 1987 Dec;25(11-12):863-79. doi: 10.1007/BF00502606.
10
Conservation and variation in the large scale organisation of the globin gene domains of duck and chicken.鸭和鸡珠蛋白基因结构域大规模组织中的保守性与变异性
Mol Gen Genet. 1986 May;203(2):193-201. doi: 10.1007/BF00333954.
雌激素和雄激素与性激素反应组织的核基质的特异性结合。
J Biol Chem. 1980 Aug 10;255(15):7265-75.
4
The loosely bound non-histone chromosomal proteins of rat prostate in androgen action.雄激素作用下大鼠前列腺中松散结合的非组蛋白染色体蛋白
Biochim Biophys Acta. 1981 Mar 26;653(1):130-8. doi: 10.1016/0005-2787(81)90111-8.
5
The effect of estrogen on the concentration of ovalbumin gene sequence in the 2 M NaCl residual fraction of oviduct chromatin.雌激素对输卵管染色质2M氯化钠残留组分中卵清蛋白基因序列浓度的影响。
FEBS Lett. 1982 Dec 13;150(1):117-21. doi: 10.1016/0014-5793(82)81316-1.
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The ovalbumin gene is associated with the nuclear matrix of chicken oviduct cells.卵清蛋白基因与鸡输卵管细胞的核基质相关。
Cell. 1982 Jan;28(1):99-106. doi: 10.1016/0092-8674(82)90379-8.
7
Human-specific nuclear protein that associates with the polar region of the mitotic apparatus: distribution in a human/hamster hybrid cell.与有丝分裂器极区相关的人类特异性核蛋白：在人/仓鼠杂交细胞中的分布
Cell. 1980 Nov;22(2 Pt 2):489-99. doi: 10.1016/0092-8674(80)90359-1.
8
Nuclear non-chromatin proteinaceous structures: their role in the organization and function of the interphase nucleus.核非染色质蛋白质结构：它们在间期细胞核的组织和功能中的作用。
J Cell Sci. 1980 Aug;44:395-435. doi: 10.1242/jcs.44.1.395.
9
Chicken reticulocyte nuclear antigen: its identification and relation to transcriptive activity in erythropoietic cells.鸡网织红细胞核抗原：其鉴定及与造血细胞转录活性的关系。
Biochemistry. 1980 May 27;19(11):2362-7. doi: 10.1021/bi00552a013.
10
Nonrandom distribution of repeated DNA sequences with respect to supercoiled loops and the nuclear matrix.重复DNA序列相对于超螺旋环和核基质的非随机分布。
Proc Natl Acad Sci U S A. 1982 Oct;79(19):5911-5. doi: 10.1073/pnas.79.19.5911.