Seth Sonali, Chakravorty Debamitra, Dubey Vikash Kumar, Patra Sanjukta
Department of Biotechnology, Indian Institute of Technology Guwahati, Assam 781 039, India.
Department of Biotechnology, Indian Institute of Technology Guwahati, Assam 781 039, India.
Protein Expr Purif. 2014 Mar;95:13-21. doi: 10.1016/j.pep.2013.11.006. Epub 2013 Nov 23.
Lipases from bacterial, fungal, and animal sources have been purified to homogeneity with very few of them being contributed from plants. Plant lipases are mostly found in energy reserve tissues, for example, oilseeds. They act as biocatalysts which are attractive due to their high substrate specificity, low production cost and easy pharmacological acceptance due to their eukaryotic origin. Hence plant lipases represent better potential for commercial applications in organic synthesis, food, detergent and pharmacological industries. However, low expression, uneconomical fold purity and the plethora of difficulties related to their recombinant expression has been limiting their commercial applicability and posing challenges to many researchers. This article focuses on comprehensive approaches that have been reported to date to address these challenges.
来自细菌、真菌和动物源的脂肪酶已被纯化至同质，而植物来源的脂肪酶却很少。植物脂肪酶大多存在于能量储备组织中，例如油籽。它们作为生物催化剂，因其高底物特异性、低成本生产以及由于其真核起源而易于被药理学接受而备受关注。因此，植物脂肪酶在有机合成、食品、洗涤剂和制药行业具有更好的商业应用潜力。然而，低表达、不经济的折叠纯度以及与其重组表达相关的诸多困难一直限制着它们的商业适用性，并给许多研究人员带来挑战。本文重点关注迄今为止报道的应对这些挑战的综合方法。
