Laboratory of Respiration Physiology, Carlos Chagas Filho Institute of Biophysics, Universidade Federal do Rio de Janeiro, São Paulo, Brazil.
Laboratory of Inflammation, Fundação Oswaldo Cruz, São Paulo, Brazil.
Respir Physiol Neurobiol. 2014 Jan 15;191:106-13. doi: 10.1016/j.resp.2013.11.004. Epub 2013 Nov 23.
We compared the toxicity of subchronic exposure to equivalent masses of particles from sugar cane burning and traffic. BALB/c mice received 3 intranasal instillations/week during 1, 2 or 4 weeks of either distilled water (C1, C2, C4) or particles (15μg) from traffic (UP1, UP2, UP4) or biomass burning (BP1, BP2, BP4). Lung mechanics, histology and oxidative stress were analyzed 24h after the last instillation. In all instances UP and BP groups presented worse pulmonary elastance, airway and tissue resistance, alveolar collapse, bronchoconstriction and macrophage influx into the lungs than controls. UP4, BP2 and BP4 presented more alveolar collapse than UP1 and BP1, respectively. UP and BP had worse bronchial and alveolar lesion scores than their controls; BP4 had greater bronchial lesion scores than UP4. Catalase was higher in UP4 and BP4 than in C4. In conclusion, biomass particles were more toxic than those from traffic after repeated exposures.
我们比较了亚慢性接触相当于甘蔗燃烧和交通产生的颗粒的毒性。BALB/c 小鼠在 1、2 或 4 周内每周接受 3 次鼻内滴注，分别用蒸馏水（C1、C2、C4）或来自交通（UP1、UP2、UP4）或生物质燃烧（BP1、BP2、BP4）的 15μg 颗粒处理。最后一次滴注后 24 小时分析肺力学、组织学和氧化应激。在所有情况下，与对照组相比，UP 和 BP 组的肺弹性、气道和组织阻力、肺泡塌陷、支气管收缩和巨噬细胞流入肺脏的情况更差。与 UP1 和 BP1 相比，UP4、BP2 和 BP4 分别出现更多的肺泡塌陷。与对照组相比，UP 和 BP 的支气管和肺泡损伤评分更差；BP4 的支气管损伤评分高于 UP4。与 C4 相比，UP4 和 BP4 中的过氧化氢酶更高。总之，重复暴露后，生物质颗粒比交通产生的颗粒毒性更大。
