Scharfstein J, Rodrigues M M, Alves C A, de Souza W, Previato J O, Mendonça-Previato L
J Immunol. 1983 Aug;131(2):972-6.
A glycoprotein of 25,000 daltons (G25) purified from T. cruzi extracts is recognized by serum antibodies of Chagas' disease patients. These human antibodies were isolated by affinity chromatography and were used to demonstrate that G25 antigenic determinants are i) represented at the parasite surface, and ii) are expressed in all developmental stages of the parasite's life cycle, as well as in several T. cruzi strains. This antigen-antibody system may be useful for the diagnosis of Chagas' disease because antibodies to radiolabeled G25 are found in the serum of 96.5% of 173 chagasic patients from different endemic areas, but are not found in the serum from other individuals. Taken collectively, the data suggest that antibodies to G25 define highly conserved determinants of the species T. cruzi. Moreover, its remarkable immunogenicity to infected humans offers an opportunity to investigate the role of specific immunologic responses in the pathogenicity of Chagas' disease.
从克氏锥虫提取物中纯化出的一种25000道尔顿的糖蛋白（G25）可被恰加斯病患者的血清抗体识别。这些人源抗体通过亲和层析法分离得到，并用于证明G25抗原决定簇：i）存在于寄生虫表面；ii）在寄生虫生命周期的所有发育阶段以及几种克氏锥虫菌株中均有表达。这种抗原-抗体系统可能对恰加斯病的诊断有用，因为在来自不同流行地区的173名恰加斯病患者的血清中，96.5%的患者血清中发现了针对放射性标记G25的抗体，而在其他个体的血清中未发现。总体而言，数据表明针对G25的抗体定义了克氏锥虫物种的高度保守决定簇。此外，它对受感染人类具有显著的免疫原性，为研究特异性免疫反应在恰加斯病致病性中的作用提供了契机。
