a Department of Coastal Sciences , University of Southern Mississippi , Ocean Springs , Mississippi , USA.
J Toxicol Environ Health A. 2013;76(21):1198-209. doi: 10.1080/15287394.2013.848394.
The BP Deepwater Horizon oil spill has great potential to negatively affect estuarine fish populations. In order to assess possible impacts of this event, a series of sublethal lab experiments were performed, using the economically and ecologically important species spotted seatrout (Cynoscion nebulosus). Larval and juvenile spotted seatrout were exposed to sublethal concentrations of high energy water accommodated fraction (HEWAF), chemically enhanced water accommodated fraction (CEWAF), or dispersant alone in an acute exposure. Response to exposure was evaluated with quantative polymerase chain reaction (qPCR) to examine expression of cytochrome P-4501A (CYP1A). Growth of larvae and juveniles over the duration of the experiment was measured as an index of physiological response. Our data showed that the different life stages respond differently to crude and dispersed oil, with larval spotted seatrout affected most by CEWAF, while juvenile spotted seatrout were affected to a greater extent by HEWAF. In both cases, the treatment with the highest CYP1A levels resulted in the greatest reductions in growth.
BP 深水地平线石油泄漏事件极有可能对河口鱼类种群产生负面影响。为了评估这一事件可能造成的影响，我们进行了一系列亚致死实验室实验，使用经济和生态上重要的物种斑点鲈（Cynoscion nebulosus）进行实验。亚致死浓度的高能水可容纳部分（HEWAF）、化学增强水可容纳部分（CEWAF）或单独的分散剂在急性暴露下暴露于幼鱼和幼鱼。通过定量聚合酶链反应（qPCR）检测细胞色素 P-4501A（CYP1A）的表达来评估对暴露的反应。在实验过程中，测量幼虫和幼鱼的生长情况作为生理反应的指标。我们的数据表明，不同的生命阶段对原油和分散油的反应不同，CEWAF 对幼鱼斑点鲈的影响最大，而 HEWAF 对幼鱼斑点鲈的影响更大。在这两种情况下，CYP1A 水平最高的处理导致生长减少最多。
