Cancer Biology Laboratory, Center of Excellence for Advanced Sciences, National Research Center, Cairo, Egypt; Department of Biochemistry, National Research Center, Cairo, Egypt.
Photodiagnosis Photodyn Ther. 2013 Dec;10(4):446-59. doi: 10.1016/j.pdpdt.2013.03.013. Epub 2013 Apr 30.
Indocyanine green (ICG) is a promising photosensitive agent for photodynamic therapy (PDT) of tumors. Encapsulating ICG dye in polymeric nanoparticles based on PEBBLE technology forming (ICG-PEBBLE) could improve the aqueous stability of the entrapped ICG molecules. The study objective is to investigate the PDT effect of free ICG-PEBBLE and its Anti-EGFR conjugate.
Skin squamous cell carcinoma was induced in CD1 mice by dimethylbenzanthracene (DMBA) and 12-O-tetradecanoyl-phorbol-13-acetate (TPA) followed by a PDT protocol for four weeks.
PDT using ICG-PEBBLE or ICG-PEBBLE-Anti-EGFR decreased skin tumor sizes. Our findings revealed that the inflammatory mediators tumor necrosis factor (TNF-α), nitric oxide (NO), cycloxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX), the angiogenic mediator vascular endothelial growth factor (VEGF), and proliferating cell nuclear antigen (PCNA) were decreased, while apoptosis, caspase-3 and histone acetylation were induced in tumor bearing groups after PDT using both of ICG-PEBBLE or ICG-PEBBLE-Anti-EGFR.
The present study indicated the effectiveness of PDT using ICG-PEBBLE or ICG-PEBBLE-Anti-EGFR as an inhibitor modality for tumor size, apoptosis, angiogenesis and tumor inflammation. The conjugating of ICG-PEBBLE to anti-EGFR was found to be more effective in inhibiting VEGF and in increasing caspase-3 compared to free ICG-PEBBLE, but there were no other preferential PDT efficacy.
吲哚菁绿（ICG）是一种很有前途的光敏剂，可用于肿瘤的光动力疗法（PDT）。基于 PEBBLE 技术将 ICG 染料封装在聚合物纳米粒子中形成（ICG-PEBBLE）可以提高包封的 ICG 分子的水稳定性。本研究的目的是研究游离 ICG-PEBBLE 及其抗 EGFR 缀合物的 PDT 效应。
通过二甲基苯蒽（DMBA）和 12-O-十四烷酰佛波醇-13-乙酸酯（TPA）在 CD1 小鼠中诱导皮肤鳞状细胞癌，然后进行四周的 PDT 方案。
使用 ICG-PEBBLE 或 ICG-PEBBLE-抗 EGFR 的 PDT 可减小皮肤肿瘤的大小。我们的研究结果表明，炎性介质肿瘤坏死因子（TNF-α）、一氧化氮（NO）、环氧化酶-2（COX-2）和 5-脂氧合酶（5-LOX）、血管生成介质血管内皮生长因子（VEGF）和增殖细胞核抗原（PCNA）减少，而在 PDT 后，肿瘤携带组中的细胞凋亡、半胱天冬酶-3 和组蛋白乙酰化被诱导。
本研究表明，使用 ICG-PEBBLE 或 ICG-PEBBLE-抗 EGFR 的 PDT 作为肿瘤大小、凋亡、血管生成和肿瘤炎症的抑制方式是有效的。与游离 ICG-PEBBLE 相比，将 ICG-PEBBLE 与抗 EGFR 缀合发现更能有效抑制 VEGF 并增加 caspase-3，但没有其他优先的 PDT 疗效。
